青藤碱对肾移植模型大鼠静脉血IL-2及CD44表达水平的影响

The Effect of Sinomenine on the Expression Levels of IL - 2 and CD44 in Serum of Rat Renal Transplantation Models

目的研究青藤碱（alkaloid sinomenine，SIN）对大鼠肾移植术后急性排斥反应的抑制作用及机制。方法84只实验动物模型分为7组：生理盐水（NS）组、SIN组、环抱菌素A（CSA）组、SIN＋CsA组、同基因移植组、假手术组、正常对照组。观察大鼠存活时间、泌尿持续时间，检测肾功能指标（sBUN、sCr）、静脉血IL-2及CD44表达水平。结果对照组大鼠长期存活，NS组均在术后9d内死亡；SIN组存活时间平均为9．39±0．73d，延长了受体鼠存活时间，SIN组、CsA组与NS组相比较，差异有显著性；SIN＋CsA组，明显延长受体鼠的存活时间至16d以上，SIN＋CsA组与NS组、SIN组、CsA组比较，差异均有显著性意义。肾功能指标：SIN、CsA均抑制受体鼠的血尿素氮（BUN）、血肌酐（Cr）增高，SIN＋CsA组的血Cr与NS组、SIN组、CsA组比较，差异有显著性意义。静脉血IL-2及CD44表达水平：除SIN组与CsA组的IL-2值相比P值〉0．05外，实验组各组间两两比较差异均有显著性意义，各实验组与各对照组间两两比较差异亦均有显著性意义。结论SIN能下调肾移植模型大鼠静脉血IL-2及CD44表达水平，从而干预参与急性排斥反应的淋巴细胞发挥作用，对大鼠肾移植术后急性排斥反应起到免疫抑制作用，并与CsA有协同作用。

Objective To evaluate the immtmosuppressive efficacy of alkaloid sinomenine （SIN） and its synergistic effect in combination with immtmosuppressant cyclosporin A （CsA） during acute rejection occurred in rat renal allograft transplantation models. Methods 84 re- cipients were divided into 7 groups at random： group NS, group SIN, group CsA, group SIN ＋ CsA, syngeneic transplantation group, sham operation group, normal controll group, qhe allografts survival time and 24 h- urinary volume were observed. Blood urea nitrogen （BUN） and creatinine （Cr） in blood were measured. The expression levels of IL- 2 and CD44 in serum were detected. Results The recipient of syngeneic transplantation group and sham operation group survived over 100 days. The recipients of group NS died within 9 days. Treatment with SIN or a subtherapeutic dose of CsA prolonged allograft survival. In contrast, SIN treatment in combination with CsA had a statistically significant synergistic efficacy, with a mean survival of 20.71 - 2.69 clays,with the 24 h- urinary volume and urinary time were also increased significandy. The blood BUN and Cr in group SIN, group CsA and group SIN ＋ CsA were lower than ones in group NS （ P 〈 0.05）, but higher than ones in other three group controls. Likewise, the expression levels of IL- 2 and CD44 in group SIN, group CsA and group SIN ＋ CsA were lower than ones in group NS （ P 〈 0.05）, but higher than ones in other three group controls. The expression levels of IL - 2 and CD44 in group SIN ＋ CsA were lower than ones in group SIN and group CsA. Conclusions SIN can decrease the expression levels of IL - 2 and CD44 in rat renal transplantation models. This may be one of its immunosuppressive mechanisms. SIN have a synergistic efficacy of anfimjection with CsA during renal allograft acute rejection.