MTBE诱导C—fos基因在中枢神经系统的表达

Expression and Distribution of C- fos Oncogene Induced by Methyl Tertiary Butyl Ether in Central Nervous System of the Rat

目的了解甲基叔丁基醚（MIBE）对中枢神经系统的作用部位，为进一步探讨MTBE对中枢神经系统的急性毒作用机制。方法雄性SD大鼠腹腔注射MIBE（0．075mL／50g）染毒1h后，应用免疫组织化学、显微图像分析技术检测Fos蛋白阳性神经元在中枢神经系统内的分布。结果7MTBE染毒组大鼠大脑皮质、下丘脑、小脑皮质均有Fos阳性神经元核团表达。结论MTBE对中枢神经系统具有明确的毒作用位点。

Objective To undetstand the distribution of C - los oncogene expression induced by MTBE in central nervous system technique （ABC method） was employed to detect C - fos oncogene. Results When toxic dose of MTBE was 0.075 ml/50g, four Fos part appeared in the central nervous system, including cerebral cortex, hypothalamus, cerebellar cortex. Condusion MTBE has the determined sites of action in central nervous system.