摘要
目的研究核酸对铅染毒大鼠淋巴细胞DNA损伤的保护作用。方法纯种雄性健康Wistar大鼠,随机分为正常对照组、铅染毒组、核酸低剂量组、核酸高剂量组。对照组正常饮水,其余3组饮0.8%醋酸铅水溶液,核酸组灌胃核酸,对照组、染毒组灌蒸馏水。5周后,采用单细胞凝胶电泳技术对各组大鼠外周血淋巴细胞DNA损伤进行分析。结果染毒组大鼠淋巴细胞DNA的迁移率和尾长显著高于对照组,核酸组大鼠淋巴细胞DNA的迁移率和尾长显著低于染毒组,在统计学上差异有显著性(P<0.05)。结论慢性铅染毒可致大鼠淋巴细胞DNA损伤,饮食核酸对淋巴细胞DNA损伤具有一定的保护作用,可减轻铅染毒所造成的氧化损伤。
Objective To observethe protective effect of Dietary Nucleic Acid on DNA damage of lymphocyte. Method The rats were given Pb and nucleic acids. After5 weeks, the DNA break in peripheral blood lymphocytes from the rats were analyzed with single cell gel electrophoresis. Results Compared with control, the damage of DNA in Dietary Nucleic Acid group were reduced significantly（ P 〈 0.05） .Conclusion Dietary Nucleic Acid could protect DNA damage of peripheral blood lymphocytes in Rats.
出处
《黑龙江医学》
2006年第2期108-109,共2页
Heilongjiang Medical Journal
关键词
核酸
淋巴细胞
DNA损伤
单细胞凝胶电泳
Nucleic Acid
Lymphocyte
DNA damage
Single cell gel electrophoresis
