青蒿琥酯诱发大鼠骨髓细胞膜系的脂质过氧化作用

Lipid peroxidation of membranes induced by sodium artesunate in rat bone marrow cells

用ＴＢＡ法测定了青蒿琥酯（Ｓｏｄｉｕｍａｒｔｅｓｕｎａｔｅ，ＳＡ）中毒大鼠骨髓脂质过氧化作用产物——丙二醛（ｍａｌｏｎａｌｄｅｈｙｄｅ，ＭＤＡ）含量以探济该药的造血毒性机制。结果表明，１００～３３３ｍｇ／ｋｇＳＡ引起骨髓ＭＤＡ含量与剂量呈正相关线性增高；相关系灵敏ｒ＝０．９５６６；骨髓ＭＤＡ增殖（ＩＭＤＡ）时间－反应曲线表明，达峰时间为１６．５ｈ，ＩＭＤＡ峰反应消除一半需７６．５ｈ。骨髓ＩＭＤＡ变化时程与骨髓细胞膜系结构损伤及造血抑制的发生、发展和恢复的时间规律基本一致。

The mechanism of sodium artesunate (SA) induced hemotoxicity was probed by TBA method to detect the product of lipid peroxidation(LPO),malonaldehyde(MDA),in in vivo SA treated rat bone marrow cells.A dose related increase of bone marrow MDA level was found at 24 h after SA 100 333 mg/kg iv.The regression coefficient was 0 956 6 The time I DMA responce curve showed that the peak responce occurred at 16 5 h and the elimination half peak response was at 76 5 h which was consistent with the couse of development of biomembrane injuries and the inhibition of hematopoiesis in SA poisoned bone marrow cells.These results seem to indicate that the drug free radical membrane LPO may be the possible mechanism of hemotoxic and genotoxic effects induced by SA.