pBLacZ真核表达载体的构建及其在体内外的表达

Construction of a Eukaryotic Expression Vector pBLacZ and Its Expression Both in Vitro and in Vivo

构建了一种编码β－半乳糖苷酶的真核基因表达载体ｐＢＬａｃＺ。用它对体外培养的ＮＩＨ／３Ｔ３、ＣＯＳ－１、ＣＨＯ细胞株、原代培养的小鼠皮肤成纤维细胞，以及小鼠活体皮下和大鼠活体骨骼肌中进行了基因转移。经Ｘ－ｇａｌ组织化学染色后的结果证实：此基因载体可在这些组织细胞中有效地表达产生β－半乳糖苷酶活性。ｐＢＬａｃＺ作为一种新的标志基因载体，可能具有一定的应用价值。

A new eukaryotic expression vector pBLacZ was constructed.It was transfected into the cell lines of NIH/3T3,COS-1,CHO and the primary cultures of murine dermatic fibroblast.We also transfected it into the murine subcutaneous layer and skeletal muscles of rats in vivo.It was detected that the gene expression vector could encode the E. Coli β-galactosidase effectively in all these histocytes.The results suggested that as a new marker gene,pBLacZ might have certain value of application.