胃旁路术对非肥胖型糖尿病大鼠的降糖作用

The hypoglycemic effects of gastric bypass in streptozotocin(STZ) -induced diabetic rats

目的:观察胃旁路术(gastricbypass,GBP)对链脲佐菌素(strept-ozotocin,STZ)诱发的非肥胖型糖尿病大鼠的降糖作用及其与术后体重、饮食量改变的关系。方法:雄性SD大鼠腹腔注射STZ建立糖尿病模型后随机分为手术组(O组)、假手术组(S组)、饮食控制组(F组)、对照组(C组),每组8只,测定术前,术后第1、2、3、4、8周各组空腹血糖、体重、平均进食量。结果:O组GBP后第3周血糖由术前的17.6±4.2mmol/L下降到13.2±3.5mmol/L(P<0.05),至实验结束血糖无反弹,S组术后与O组保持相似的体重改变,血糖无明显下降;F组控制平均进食量约为O组的1/3并导致显著体重下降,血糖下降没有O组明显(P<0.05),术后第3周起O组的平均进食量少于S组和C组(P<0.05)。结论:GBP能显著降低STZ诱导的非肥胖型糖尿病大鼠血糖,并与GBP后大鼠体重下降及饮食减少无关。

Objective： To observe the hypoglycemic effects of gastric bypass（GBP） in streptozotocin（STZ）-induced diabetic rats and to investigate the relationship between gastric bypass and weight loss or decreased food intake. Methods：Thirty-two male diabetic SD rats induced by STZ were randomly allocated into four groups：GBP （group O）, shame operation （group S）, food restriction （group F） and controls（group C）. Plasma glucose concentration, weight and food intake were measured before operation and 1 week, 2 weeks, 3 weeks, 4 weeks, 8 weeks after operation in all rats. Results： The plasma glucose lever decreased from 17.6 ± 4.2 mmol/L before operation to 13.2 ± 3.5 mmol/L at the time of 3 weeks after GBP, and remained in low level until the end of the experiment. GBP resulted in better glycemic control,compared with food restriction （P 〈 0.05）. GBP rats showed less average daily food intake than rats undergoing sham operation or food restriction since 3 weeks after operation （P 〈 0.05）. Conclusion：GBP can significantly reduce plasma glucose in STZ-diabetic rats, and the hypoglycemic effects are not associated with weight loss or lower food intake.