栀子提取物中大孔吸附树脂残留物的测定

Determination of residuum from gardenia extract by macroporous adsorption resin

目的：建立测定栀子提取物中大孔吸附树脂残留甲苯、二甲苯、二乙烯基苯与苯乙烯含量的方法。方法：使用气相色谱法，采用DB．INNOWax毛细管柱，以氮气为载气．FID检测器，程序升温技术。结果：栀子提取物样品中，甲苯〈890mg／L，二甲苯〈2170mg／L,二乙烯基苯与苯乙烯均〈2000mg／L；在所考察的浓度范罔内线性关系良好，平均回收率在96．8％-104．4％，定量限为0．125—2．87mg／l。结论：该方法简便、灵敏度高，重复性好，结果准确，可用于栀子提取物的质量控制。

AIM ： To establish the method of deternlining methylbenzene ,dimethylbenzene, divinylbenzene, and phenylethene in gardenia extract. METHODS： The GC system consisted of DB-INNOWax capillary chromatographic column, nitrogen as the carrier gas, and FID as the detector. RESULTS： Methylbenzene was less than 890 mg/L, dimethylbenzene 2 170 mg/L, divinylbenzene and phenylethene 2000 mg/L, vespectively in gardenia extract. The average recoveries were within 96.8% - 104.4%. The limits of detection were 0. 125 mg/L -2.87 mg/L. CONCLUSION： The method is sensitive, accurate and reproducible, and it can be used to control the quality of gardenia extract.