芳樟树叶乙醇提取物的抗癌作用

Antitumor action of ethanolic extractives from camphor leaves

探讨了芳樟树叶乙醇提取物各部分的抗癌作用。采用系统溶剂法从芳樟树叶得到一系列提取物,运用MTT和集落形成实验法考察各提取物对体外培养的人肺癌95-D细胞、人口腔表皮样癌KB细胞和肝癌HepG2细胞增殖的影响。结果发现石油醚部分A1、氯仿部分A2和乙酸乙酯部分A3对3种肿瘤细胞的增殖和集落形成均有不同程度的抑制作用,且给药量与细胞抑制和集落形成明显的正相关关系。因此,芳樟树叶乙醇提取物在体外有明显的抗肿瘤作用,且抗癌有效组分很可能在它的石油醚部分、氯仿部分以及乙酸乙酯部分。

The in vitro antitumor action of the different parts of ethanolic extractives from Camphor leaves, which were obtained by the technique of solvent-refining, was investigated in this paper. The dried Camphor leaves were submitted to ethanol （95%v/v） extraction and then the ethanolic extract was extracted again by petrol-ether, chloroform, ethyl acetate, methanol and water successively, and five extractives - A1, A2, A3, A4 and A5, were attained correspondingly. Using MTT and colony-forming methods, the five extractives were tested for the cytotoxicity on high metastatic lung carcinoma cells （95-D）, human oral epidermoid carcinoma （KB） and the human hepatoma cell line （HepG2）. The extrac- A1, A2 and A3, showed the significant antitumor action when compared with the controls. The three extractives inhibited the tumor cells viability in concentration- dependent characteristics. These investigations suggested that the extract from camphor leaves had the function of antitumor in vitro, which was reported here firstly.