气血并治方及其配伍药对对高脂血症血瘀大鼠血小板聚集性和血小板活化表达率的影响

Effects of Qi-blood Symphyso-treated Formula and Its Herb-pairs on Platelet Aggregation and Expression of Platelet Activated Molecule in Rats with Hyperlipemia

目的:研究观察气血并治方及其方中配伍药对对高脂血症大鼠脂质代谢、血小板聚集性、血小板活化分子表达率的影响,比较它们作用强度和作用环节的差异。方法:采用高脂饲料和应激(肾上腺素)造成大鼠高脂血症血瘀模型,灌胃给药,观察全方及方中活血药药对、理气药药对对高脂血症血瘀动物模型的影响。结果:全方组、川芎-赤芍组、柴胡-赤芍组及柴胡-枳壳组对大鼠总胆固醇(TC)和低密度脂蛋白(LDL-C)均有显著降低作用(P<0.05,P<0.01);全方组对甘油三酯(TG)和脂蛋白(α)也有显著降低作用(P<0.05);柴胡-赤芍组及柴胡-枳壳组对脂蛋白(α)有显著降低作用(P<0.05)。全方组、川芎-赤芍组对血小板聚集性有显著抑制作用(P<0.05);柴胡-赤芍组、柴胡-枳壳组对血小板活表达率有显著抑制作用(P<0.05)。结论:气血并治方及方中活血药药对和理气药药对可作用于动物高脂血症模型的不同环节,二者配伍后的疗效在某些方面有一定增加。

Objective： To observe the effects of qi-blood symphyso-treated formula and its herb-pairs on fat metabolism, platelet aggregation and expression of platelet activated molecule in rats with hyperlipemia, compare the differences of their effect intensity and effect component element. Methods ： Hyperlipemia blood-stasis rat models were set up by high lipoids feedstuff and stress （adrenalin）. The effects of full formula and its promoting blood flow and regulating vital energy herb-pairs on Hyperlipemia blood-stasis rat models were observed after intragastric administration. Results： Cholesterol total （TC） and low density lipoprotein （LDL-C） in full formula group, szechwan lovage rhizome and red peony root group, bupleurum root and red peony root group and bupleurum root and orange fruit group were markedly degraded （ P 〈 0. 05, P 〈 0. 01 ） ; Triglyceride （TG） and lipoprotein （α） in full formula group were also notably declined （P 〈 0. 05 ） ; Lipoprotein （α） in bupleurum root and red peony root group and bupleurum root and orange fruit group were markedly degraded （P 〈 0. 05 ） . Platelet aggregation in full formula group and szechwan lovage rhizome and red peony root group were notably refrained （ P 〈 0. 05） ; Platelet activated rate in bup- leurum root and red peony root group and bupleurum root and orange fruit group were markedly restrained （ P 〈 0. 05 ）. Conclusion： Qi-blood symphyso-treated formula and its promoting blood flow and regulating vital energy herbpairs have effects on different component dements of hyperlipemia animal models; after compatibility of the two herbpairs, the therapeutic effects have certain increases in some aspects.