高效液相色谱法测定丹栀逍遥丸中栀子苷的含量

Determination of Gardenoside in Dangzixiaoyao Capsules by HPLC

目的采用高效液相色谱法洲定丹栀逍适丸中扼子苷的舍量。方法色谱柱为Kromasil C18（150×4．6mm·5μm），流动相为；乙腈。水（15：85）；流速：1．0ml．min^-1．检测波长为238nm。结果栀子苷的进样量在0．30～3．00pg范围内与峰面积呈起好线形（r=0.9994）；平均回收率为98．96％，RSD为1．32％（n=5）。结论该法简便，结果可靠．可有效地用于丹扼逍遥丸的质量控制。

OBJECTIVE The content of gardenoside in Dangzixiaoyao Capsules was determined by HPLC. METHODS The chromatographic column was Kromasil C18. The mobile phase was acetonitrile-water（15 ： 85）. The flow rate was 1.0ml · min^-1 and the detection wavelength was 238nm. The external standard and peak area method were used to determine gardenoside content. RESULTS There was a good linear relationship between gardenoside and peak area when the sample injection was from 0.30- 3.00μg. The average recovery of adding sample was 98.96% and RSD was 1.32%（n=5）. CONCLUSION This method is simple and believable. It can be effectively used for the quality control of Dangzixiaoyao Capsules.