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Β-CATENIN和COX-2在大鼠毛囊隆突区细胞中的表达及意义 被引量：1

Expression and significance of β-catenin and cox-2 in bulge-originated cells from rat hair follicles
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摘要 目的通过观察Β-CATENIN和COX-2在体外培养的毛囊隆突区细胞中的表达,探讨其对隆突区细胞增殖的作用及相互关系。方法在体外分离培养毛囊隆突区细胞的基础上,采用免疫细胞化学方法分别检测不同时相Β-CATENIN和COX-2在毛囊隆突区细胞中的表达。结果Β-CATENIN和COX-2在毛囊隆突区细胞呈强表达,并表现出与培养时间的相关性;同时Β-CATENIN可见于细胞质与细胞核中,而COX-2仅见于细胞核。结论Β-CATENIN和COX-2对毛囊隆突区细胞具有促增殖作用,COX-2可能是Β-CATENIN途径发挥作用的核内靶基因。 Objective To study the expression of and investigate relationship of their expression on ce β-catenin and cox-2 in the bulge cells of hair follicle 11 proliferation. Methods The hair follicle was prepared from the resected cheek skin of 20 Wistar rats aged 7 days. The bulge cells were resected from the intact hair follicle and cultured in vitro. Immunocytochemical technique （ ICC ） was applied to detect β-catenin and cox-2 expression in bulge cells at culture day 3, 5, 8, 13. Results β-catenin and cox-2 strongly expressed in bulge while cells and the expression correlated with the culture days. β-catenin appeared in both plasma and nucleus, cox-2 only in nucleus. Conclusion β-catenin and cox-2 were correlated with bulge cells proliferation, and cox-2 might be the target gene of β-catenin signaling pathway in nucleus.
作者 张艺 王韵 曾益军 杨恬
机构地区 第三军医大学基础医学部细胞生物学教研室
出处 《第三军医大学学报》 CAS CSCD 北大核心 2006年第4期324-326,共3页 Journal of Third Military Medical University
基金 国家自然科学基金资助项目(30371383 30471568) 第三军医大学科研及创新基金资助项目(2003-38)~~
关键词 Β-连环蛋白 环氧合酶-2 毛囊 隆突区 增殖 β-catenin cox-2 hair follicle bulge proliferation
分类号 R322.995 [医药卫生—人体解剖和组织胚胎学] R329.26 [医药卫生—人体解剖和组织胚胎学]
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参考文献9

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同被引文献14

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  • 8Sohn K C, Shi G, Jang S, et al. Pitx2, Pitx2, a beta-catenin-regulated transcription factor, regulates the differentiation of outer root sheath cells cultured in vitro[J]. J Dermatol Sci, 2009, 54( 1 ) :6 -11.
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第三军医大学学报
2006年 第4期

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