P53水平对端粒酶逆转录酶表达的调节作用

Regulatory effect of p53 on human telomerase reverse transcriptase expression

目的研究野生型P53表达水平对端粒酶逆转录酶(HTERT)表达的调节作用。方法采用针对P53的反义核酸转染HEPG2细胞和稳定表达PPUR/U6/HTERT的HEPG2细胞,野生型P53表达质粒转染HEPG2细胞,以WESTERN-BLOT验证其对靶基因的抑制或增强作用,然后再用WESTERN-BLOT检测HTERT的表达水平。结果P53反义核酸和野生型P53表达质粒能明显降低或增强P53的表达,降低P53的表达对HTERT的表达无明显影响;而P53表达增加则可抑制HTERT的表达。结论P53与HTERT之间存在相互作用,野生型P53对HTERT的表达起负调节作用。

Objective To investigate whether p53 expression is involved in human telomerase reverse transcriptase （hTERT） regulation. Methods HepG2 cells and pPUR/U6/hTERT HepG2 cells were treated with the p53 antisense oligonucleotide respectively and p53 expression plasmid were introduced to HepG2 cells. The decreased or increased p53 level and the hTERT expression levels were analyzed by Western blotting. Results Down-regulation of p53 expression had no obvious effect on hTERT expression, but over-expression of p53 could significantly repress hTERT level. Conclusion p53 interacts with hTERT and inhibits hTERT expression in HepG2 cells.