摘要
目的对神经干细胞向神经元定向分化过程中微管蛋白的表达变化进行光、电镜观察研究。方法采用细胞培养技术、免疫荧光技术以及免疫电镜技术对神经干细胞向神经元定向分化过程中微管蛋白的表达变化进行观察。结果在神经干细胞向神经元定向分化的不同时期,存在微管蛋白的表达变化,在分化初期以核周附近分布明显,随神经元的成熟散在分布于胞质中及突起内,形成细网状,构成细胞骨架,维持细胞形态。结论在神经干细胞向神经元定向分化过程中伴随有微管蛋白的表达变化,随神经元的成熟而构成细胞骨架,维持细胞形态。
Objective To observe and study the expression changes of β-tubulin in neuron-oriented differentiation process of neural stem cells with optical and electron microscopes. Methods Using the techniques of cell culture, fluorescent immunocytochemistry and immunoelectron microscopy, we observed the expression changes of tubulin in neuron-oriented differentiation process of neural stem cells. Results During the different phases of the differentiation process there were expression changes of tubulin: in the early phase it showed an obvious distribution around nuclei, and then, with the maturation of neurons, it was scattered in cytoplasm and processes to form thin network, composing the cell skeleton and sustain the cell shape. Conclusion Expression changes of tubulin are concomitant with the neuron-oriented differentiation process of neural stem cells, and, with the maturation of neurons, it composes the cell skeleton and sustain the cell shape.
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
2006年第1期13-17,共5页
Chinese Journal of Histochemistry and Cytochemistry
基金
国家自然科学基金资助(30070371)
关键词
神经干细胞
定向分化
微管蛋白
Neural stem eell
Oriented differentiation
β-tubulin