人MII期卵母细胞纺锤体与正常受精卵早期卵裂的关系

The relationship between the early cleavage and human MII oocytes' spindles

目的探讨早期卵裂与胚胎质量及MⅡ期人卵纺锤体的关系。方法于卵泡浆内单精子注射（ICSI）后25～27h观察卵裂情况。若此时出现了卵裂就认为该受精卵发生了早期卵裂（EC）。比较EC组与无早期卵（NEC）裂组的良好胚胎形成率以及EC与MⅡ期卵母细胞纺锤体的关系。结果EC与纺锤体存在与否的r2值为0．422，P=0．000。认为EC与MⅡ期卵母细胞纺锤体存在呈正相关。EC组的良好胚胎形成率（86．4％）高于NEC组（43．8％），差异有显著性（P=0．001）。结论出现EC的胚胎D3天良好胚胎形成率高。EC与纺锤体存在与否有较为密切的关系。

Objective： To analyze the relationship between the early cleavage and the quality of embryos, and to study whether the existence of the MⅡ oocyte＇s spindle predicts the early cleavage of the normal fertilized oocyte. Methods： The embryos that possessed two cells at 25-27 hrs post ICSI were designated as early cleavage （EC） embryos and those that had not yet cleaved were classified as no early cleavage （NEC） embryos. The high quality embryos rates were compared in the EC group and the NEC group. Results： The early cleavage and the existence of spindle were positively related （rs=0. 422, P=0. 000）. The difference of high quality embryo rate between the EC group and the NEC group was statistically significant （P=0 001）. Conclusion： The early cleavage may have a predictive value on the development to the high quality embryos. The existence of the spindle is closely related to the early cleavage.