利用光谱差异的荧光法同步测定儿茶酚胺肾上腺素和去甲肾上腺素

SIMULTANEOUS DETERMINATION OF CATECHOLAMINE, EPINEPHRINE AND NOREPINEPHRINE BY SYNCHRONOUS FLUOROMETRY UTILIZING THE DIFFERENCE OF SPECTRA

本文介绍了一个利用肾上腺素(E)和去甲肾上脲素(NE)光谱之间的差异同步测定儿茶酚胺(CA)、E和NE的荧光法。它不需要对CA混合物进行复杂的分离,即可在同一溶液中进行测定。它是通过铁氰化钾氧化CA生成三羟吲哚,利用锌离子提高其荧光强度到原来的4倍左右以提高方法的灵敏度和准确度。血浆CA(以E计)、E和NE的平均回收率分别为95.2％、89.1％和70.93％。尿的平均回收率分别为64.2％,59.4％和51.67％。灵敏度分别为0.1ng/ml、0.35ng/ml和0.5ng/ml。血浆的CA、E和NE的批内变异系数分别是6.2％,7.8％和9.5％(n=4);批间变异系数分别是11.3％、12.8％和14.5％(n=3)。尿液CA、E和NE的批内变异系数分别是5.9％、8.4％和9.6％(n=4);批间变异系数分别是11.8％、12.9％和17.8％(n=3)。测定45例正常人的血浆结果是((?)±SD):CA5.55±5.40ng/ml;E4.35±4.75ng/ml;NE1.78±2.74ng/ml。20例正常人24h尿的结果是((?)±SD);CA15.11±8.35ug/24h;E9.41±5.46ug/24h;NE11.83±6.69ug/24h。

A new modified fluorometric determination for catecholamine (CA), epinephrine (E) and norepinephrine (NE) was reported. The method did not need the complicated seperate procedure of CA mixture and might determinate CA, E and NE simultaneously in the same solution, utilizing the difference of spectra between E and NE.It based upon the principle that the catecholamine (include E and NE) can be quantatively determinated through oxidation by potassium ferricyanide to produce a fluorecsent substance—trihydroxyindol, the sensitivity and accuracy of the method enhanced while the intensity of fluorecsence has been increased about 4—fold by zincic ion. The plasma CA (in term of E), E and NE recovery rate averaged about 95.2％, 89.1％ and 70.93％ respectively. The minimal measurable CA, E and NE amount of this method was 0.1ng/ml, 0.35ng/ml and 0.5ng/ml respectively. The coefficient of variation (CV) in plasma (n=4) within one batch was about 6.2％, 7.8％ and 9.5％ respectively, and the CV among different batches was about 11.3％, 12.8％ and 14.5％ respectively (n=3). The CV in urine (n=4) within one batch was about 5.9％, 8.4％ and 9.6％ respectively, the CV among different batches was about 11.8％, 12.9％ and 17.8％ respecitvely (n=3). We determinated with our method the plasma CA, E and NE in 45 ostensibly healthy humans, the concentration was about 5.5±5.4, 4.35±4.75 and 1.78±2.74 (ng/ml) respectively ((?)±SD). A normal range study on 24 hours urine was carried out in 20 ostensibly healthy humans, the total CA, E and NE excretion was about 15.11±8.35, 9.41±5.46 and 11.83±6.69(ug/ml) erspectively ((?)±SD).