白细胞介素12在大鼠颈部心脏移植排斥反应中的表达及意义

Expression and clinical significance of IL-12 in acute cardiac allograft rejection in rats

目的探讨白细胞介素12(IL-12)在心脏移植排斥反应中的表达及其与排斥反应的关系。方法采用大鼠颈部心脏移植模型,随机分为2组:同基因移植组,供、受体均为SD大鼠;异基因移植组,供、受体分别为W istar、SD大鼠。以β-Actin作内参照,分别于术后第1,3,5,7天取移植心脏,用逆转录聚合酶链式反应(RT-PCR)法观察IL-12的表达情况。结果随着术后天数的增加,异基因移植组IL-12表达逐渐升高,第7天达高峰(与对照组相比,P<0.01)。结论IL-12的表达与心脏移植急性排斥反应的发生发展密切相关,可作为心脏移植急性排斥反应的监测指标,对急性排斥反应的早期诊断和移植物的预后估计具有重要的临床意义。

Objective To explore the expression and clinical significance of IL-12 in acute cardiac allograft rejection in rats. Methods Two groups of SD rats received cervical heterotopic heart transplantation from allogeneic Wistar or syngeneic SD rats. The cardiac grafts were harvested at 1,3, 5, and 7 days after transplantation and detected for the expression of IL-12 mRNA by reverse transcriptional polymerase chain reaction （RT-PCR）. Results With the post-transplantation day delayed, the expression of IL-12 mRNA was significantly increased in allogeneic transplant group （ P 〈 0. 01 ）. Conclusion The expression of IL-12 is highly related with the development of acute cardiac allograft rejection in rats and may be used as monitoring index in acute rejection because of its important clinical values in early diagnostic of acute rejection and predicting the prognosis of allograft.