中国龙虾变应原性组分分析

Analysis on the Allergen Composition in Panulirus stimpsoni

[目的]对中国龙虾(Panulirusstimpsoni)变应原性组分进行分离和鉴定。[方法]用Coca's液提取法制备龙虾变应原粗浸液,经聚丙烯酰胺凝胶电泳(SDS-PAGE)分离蛋白质组分,并用凝胶成像系统测定各组分的相对分子质量;采用蛋白质-印迹(Western-blotting)鉴定龙虾的主要及次要变应原。[结果]中国龙虾变应原粗浸液分离得到14种以上的蛋白质组分,有6种蛋白质能与患者血清特异性IgE结合,其中分子量为23000、34000、64000的蛋白质结合率较高。[结论]中国龙虾分子量为23000、34000和64000的蛋白质为主要变应原,分子量26000和64000两种蛋白质在粗浸液中含量并不高,但变应原性较强。

[ Objective ] To isolate and identify the allergic composition of Panulirus stimpsoni. [ Methods ] Panulirus stimpsoni crude extract was prepared in Coca＇ s solution and then electrophorcsed on SDS polyacrylamide gel （ SDS-PAGE ）. Relative molecular weight of each protein band was determined with Gel Media System. The primary and secondary allergen proteins were identified by Western-blotting. [ Results ] More than fourteen protein bands from Panulirus stimpsoni chide extract were obtained. Six of protein bands can be conjugated with the specific IgE in sera of known Panulirus stimpsoni allergic patients. These protein bands with relative molecular weight of 23 000, 34 000 and 64 000 had higher binding potency. [ Conclusion ] The primary allergens of Panulirus stimpsoni are the proteins with relative molecular weight of 23 000, 34 000 and 64 000.