γ射线照射诱导大鼠肝细胞线粒体自噬与再生的研究

Study on γ-ray induced mitochondrial autophagy and regeneration of liver cells in rats

目的探讨线粒体自噬在细胞损伤后再生中的作用。方法在体外利用γ射线照射诱导大鼠肝细胞损伤,在亚细胞水平上观察肝细胞的损伤修复情况与线粒体自噬的关系,并利用分子生物学技术进行DNA凋亡片段的分析和线粒体RNA的RT-PCR分析。结果受损线粒体清除过程存在线粒体自噬,随着修复过程的进行细胞DNA凋亡程度下降,细胞自噬现象减少,细胞凋亡和线粒体自噬同时存在于损伤细胞中,通过RT-PCR分析发现肝组织的损伤修复与线粒体自噬后的线粒体再生有关。结论线粒体自噬可能为组织的损伤修复提供某种分子信号,促使新的线粒体再生。

Objective To explore the role of mitochondrial autophagy in wound healing. Methods Damages of liver cells in rats after γ-ray irradiation with a total dose of 8 Gy were investigated. The relationship between mitoehondrial autophagy and repair of liver cells, was observed uhrastrueturally. Apoptosis-related DNA fragment and mitoehondrial RNA levels were analyzed. Results Most of the damaged mitochondria were removed through mitoehondrial autophagy. Apoptosis-related DNA fragment analysis implied that, accompanied with reduction of mitoehondrial autophagy, the amount of cellular apoptosis was decreased during wound healing. Wound healing was related to mitoehondrial regeneration after mitoehondrial autophagy. Conclusion Mitochondrial autophagy may release some molecular signals for wound healing so as to accelerate sufficiently the regeneration of newly formed mitoehondria.