TRIO的扩增和高表达与膀胱肿瘤细胞的快速增殖有关

TRIO amplification and over-expression is associated with rapid tumor cell proliferation in urinary bladder cancer

目的TRIO基因位于染色体5p扩增区、编码1种在细胞周期调节中起主导作用的蛋白,本研究旨在了解TRIO的扩增和表达是否与膀胱肿瘤细胞的快速增殖有关。方法Ki67标记染色(Ki67LI)、荧光原位杂交(FISH)和定量PCR法(LightCyclerTMPCR)被用于研究。结果TRIO基因扩增与膀胱肿瘤细胞的快速生长相关(P<0.0001)。膀胱肿瘤分化越差、临床分期越高,Ki67LI与TRIO基因扩增数目均越高。含2317例组织微阵列FISH结果显现TRIO扩增仅见于1.8%早期膀胱肿瘤(pTa)(9例/499例),而在膀胱癌(pT1-4)高达12.8%(62例/485例)。定量PCR法进一步证实TRIO在显示5p扩增的膀胱癌组织高表达。结论TRIO扩增与膀胱肿瘤细胞的快速增殖有关,TRIO扩增和高表达可能在膀胱癌的发展过程中发挥重要作用。

Objective：TRIO gene located to human chromosome bands 5p encodes a protein with a putative role in cell-cycle regulation. This study aims to find out whether the amplification and expression of TRIO is correlated to rapid proliferation of bladder cancer cells. Methods： Ki 67 labeling index（Ki-67LI）, fluorescence in situ hybridization（FISH）, and quantitative PCR （LightCycler TMPCR） were used. Results：TRIO amplification was strongly associated with rapid proliferation of bladder cancer cells（P〈0.0001）. High tumor grade and low tumor differentiation was accompanied with elevated Ki67 1.1 and TRIO amplification. Tissue microarray FISH results containing 2 317 samples showed that TRIO amplification was observed only in 9 of 499 pTa tumors （1.8%） but 62 of 485 pT1-4 carcinomas （12.8%）. Quantitative PCR analysis confirmed that TRIO was over-expressed in 5p-amplified bladder tumors. Conclusion： TRIO amplification was associated with rapid tumor cell proliferation in blad der cancer. TRIO amplification/over-expression has a potential role in bladder cancer progression.