宫颈腺癌组织Skp2与p27^(kip1)表达及临床意义

Expression of Skp2 and p27(kip1) in cervical adenocarcinoma

目的研究宫颈腺癌组织中S期激酶相关蛋白2(Skp2)和细胞周期调控因子p27kip1的表达及其临床意义。方法采用组织微阵列技术结合免疫组化(二步法)检测106例宫颈腺癌组织和22例慢性宫颈炎组织中Skp2和p27kip1的表达。结果106例宫颈腺癌组织Skp2阳性表达率为67.9%,显著高于慢性宫颈炎组织(P<0.01);p27kip1阳性表达率为58.5%,明显低于慢性宫颈炎组织(P<0.05)。Skp2在宫颈腺癌的病理分级中,G2、G3组阳性表达率均明显高于G1组(P<0.05)。Skp2和p27kip1表达与宫颈腺癌组织学类型有明显相关性,子宫内膜样腺癌Skp2阳性表达率明显高于其他类型宫颈腺癌(P<0.05),透明细胞腺癌p27kip1阳性表达率明显低于其他类型宫颈腺癌(P<0.05)。Skp2和p27kip1阳性表达率无明显负相关。结论Skp2高表达与p27kip1低表达在宫颈腺癌的发生、发展中起重要作用,Skp2高表达可作为宫颈腺癌恶性化的分子指标;采用组织微阵列进行Skp2和p27kip1免疫组化检测具有可靠性和实用性。

Objective： To test the expressions of S phase kinase-associated protein 2 （Skp2） and cell cycle regulator p27 （kipl） in cervical adenoearcinoma. Methods： The expressions of Skp2 and p27 （kipl） were determined by tissue microarray combined with immunohistochemistry in 106 cases of cervical adenocarcinoma and 22 cases of cervical chronic inflammation. Results： The positive rate of Skp2 in cervical adenocarcinoma was 67.9%, which was significantly higher than that in cervical chronic inflammation（P〈0.01）. The positive rate of p27 （kipl） in cervical adenocarcinoma was 58.5%, which was markedly lower than that in cervical chronic inflammation（P〈0.05）. The positive expression of Skp2 was significantly higher in G2 and G3 stage than in G1 stage of cervical adenocareinoma（P〈0.05）. The expressions of Skp2 and p27（ kipl ） were related to the histologie classification. The level of Skp2 was significantly higher in endometrioid adenocarcinoma than that in other types（P〈0.05）. The level of p27（kipl） was significantly higher in clear-cell adenocarcinoma than that in other types. There was no obviously negative correlation between the expression of Skp2 and p27 （kipl） in cervical adenocarcinoma. Conclusions：High expression of Skp2 and low expression of p27 （kipl） play an important role in the progression of cervical adenocarcinoma. Skp2 might be the one of biomoleeular markers of malignancy for cervical adenocarcinoma. Tissue microarray is reliable and practical to detect the expressions of Skp2 and p27 （kipl）.