丹参对重症急性胰腺炎内皮素-1 mRNA的影响

Effect of Salviae Miltiorrhizaeon expression of endothelin-1 mRNA in rats with severe acute pancreatitis

目的:探讨内皮素-1(ET-1)在重症急性胰腺炎(severeacutepancreatitis,SAP)胰腺中的表达对胰腺损伤的作用机制及丹参注射液对ET-1mRNA表达的影响.方法:Wistar大鼠45只,随机等分为模型组、药物组和对照组.以50g/L牛磺胆酸钠1mL/kg经胰管内逆行注射复制SAP模型.药物组在制模后im丹参[2mL/(kg·d)],1次/6h.对照组仅行假手术.各组动物在术后24h测血淀粉酶、ET-1和腹水量.使用原位杂交和图象分析检测胰腺ET-1mRNA的表达强度,并观察胰腺的病理变化.结果:模型组血中淀粉酶和腹水量分别为35.9±5.93μkat/L和9.87±2.34mL,显著高于药物组的23.96±5.56μkat/L和5.27±2.81mL及对照组的3.60±0.62μkat/L和0mL(P<0.001).模型组血中ET-1为185.47±20.80ng/L,高于药物组的164.27±18.53ng/L和对照组的72.90±17.27ng/L(P<0.05,P<0.001).模型组和药物组胰腺ET-1mRNA表达均增高,模型组显著高于药物组(28.22±1.15vs23.81±1.04,P<0.001).模型组和药物组胰腺均有病理改变,但药物组较模型组减轻(评分:10.45±1.42vs12.05±1.28,P<0.05).结论:SAP时胰腺ET-1mRNA高表达导致ET-1过度生成并与胰腺损伤有关.丹参能抑制胰腺ET-1mRNA的过度表达,从而对胰腺起保护作用.

AIM： To investigate the expression of endothelin-1 （ET-1） mRNA in severe acute pancreatitis （SAP） as well as the effect of Salviae Miltiorrhizae （SM） on ET-1 mRNA expression in rats. METHODS： Forty-five Wistar rats were randomly divided into 3 groups： model group （MG, n = 15）, SM group （SMG, n = 15）, and control group （CG, n = 15）. Severe acute pancreatitis was induced by intraductal injection of 50 g/L sodium taurocholate （1.0 mL/kg） in MG and SMG. The rats in SMG were then treated with Salviae Miltiorrhizae [2 mL/（kg · d）]. Sham-operation was only performed in CG. The levels of ascites, serum ET-1 and amylase （AMY） were determined at 24 h. The expression of ET-1 mRNA was examined by in situ hybridization in pancreatic tissues. The pathological changes of the pancreas were observed 24 h after modeling. RESULTS： The levels of ascites and serum AMY in MG （9.87 ± 2.34 mL, 35.9 ± 5.93 μkat/L） and SMG （5.27 ± 2.81 mL, 23.96 ± 5.56 μkat/L） were markedly higher than those in CG （0 mL, 3.60 ± 0.62 μkat/L, P 〈 0.001）. The level of serum ET-1 in MG was significantly higher than that in SMG and CG （185.47 ± 20.80 ng/L vs 164.27 ± 18.53 ng/L, 72.90 ± 17.27 ng/L, P 〈 0.05, P 〈 0.001）. ET-1 mRNA expression was notably higher in MG than that in SMG （28.22 ± 1.15 vs 23.81 ± 1.04, P 〈 0.001）. Pathological changes were obviously alleviated in SMG in comparison with those in MG （score： 10.45 ± 1.42 vs 12.05 ± 1.28, P 〈 0.05）. CONCLUSION： Over-expression of ET-1 mRNA in pancreas plays an important role in SAP, and SM can inhibit the expression of ET-1 mRNA and alleviate the pancreatic injury.