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丹参对重症急性胰腺炎内皮素-1 mRNA的影响 被引量:16

Effect of Salviae Miltiorrhizaeon expression of endothelin-1 mRNA in rats with severe acute pancreatitis
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摘要 目的:探讨内皮素-1(ET-1)在重症急性胰腺炎(severeacutepancreatitis,SAP)胰腺中的表达对胰腺损伤的作用机制及丹参注射液对ET-1mRNA表达的影响.方法:Wistar大鼠45只,随机等分为模型组、药物组和对照组.以50g/L牛磺胆酸钠1mL/kg经胰管内逆行注射复制SAP模型.药物组在制模后im丹参[2mL/(kg·d)],1次/6h.对照组仅行假手术.各组动物在术后24h测血淀粉酶、ET-1和腹水量.使用原位杂交和图象分析检测胰腺ET-1mRNA的表达强度,并观察胰腺的病理变化.结果:模型组血中淀粉酶和腹水量分别为35.9±5.93μkat/L和9.87±2.34mL,显著高于药物组的23.96±5.56μkat/L和5.27±2.81mL及对照组的3.60±0.62μkat/L和0mL(P<0.001).模型组血中ET-1为185.47±20.80ng/L,高于药物组的164.27±18.53ng/L和对照组的72.90±17.27ng/L(P<0.05,P<0.001).模型组和药物组胰腺ET-1mRNA表达均增高,模型组显著高于药物组(28.22±1.15vs23.81±1.04,P<0.001).模型组和药物组胰腺均有病理改变,但药物组较模型组减轻(评分:10.45±1.42vs12.05±1.28,P<0.05).结论:SAP时胰腺ET-1mRNA高表达导致ET-1过度生成并与胰腺损伤有关.丹参能抑制胰腺ET-1mRNA的过度表达,从而对胰腺起保护作用. AIM: To investigate the expression of endothelin-1 (ET-1) mRNA in severe acute pancreatitis (SAP) as well as the effect of Salviae Miltiorrhizae (SM) on ET-1 mRNA expression in rats. METHODS: Forty-five Wistar rats were randomly divided into 3 groups: model group (MG, n = 15), SM group (SMG, n = 15), and control group (CG, n = 15). Severe acute pancreatitis was induced by intraductal injection of 50 g/L sodium taurocholate (1.0 mL/kg) in MG and SMG. The rats in SMG were then treated with Salviae Miltiorrhizae [2 mL/(kg · d)]. Sham-operation was only performed in CG. The levels of ascites, serum ET-1 and amylase (AMY) were determined at 24 h. The expression of ET-1 mRNA was examined by in situ hybridization in pancreatic tissues. The pathological changes of the pancreas were observed 24 h after modeling. RESULTS: The levels of ascites and serum AMY in MG (9.87 ± 2.34 mL, 35.9 ± 5.93 μkat/L) and SMG (5.27 ± 2.81 mL, 23.96 ± 5.56 μkat/L) were markedly higher than those in CG (0 mL, 3.60 ± 0.62 μkat/L, P 〈 0.001). The level of serum ET-1 in MG was significantly higher than that in SMG and CG (185.47 ± 20.80 ng/L vs 164.27 ± 18.53 ng/L, 72.90 ± 17.27 ng/L, P 〈 0.05, P 〈 0.001). ET-1 mRNA expression was notably higher in MG than that in SMG (28.22 ± 1.15 vs 23.81 ± 1.04, P 〈 0.001). Pathological changes were obviously alleviated in SMG in comparison with those in MG (score: 10.45 ± 1.42 vs 12.05 ± 1.28, P 〈 0.05). CONCLUSION: Over-expression of ET-1 mRNA in pancreas plays an important role in SAP, and SM can inhibit the expression of ET-1 mRNA and alleviate the pancreatic injury.
出处 《世界华人消化杂志》 CAS 北大核心 2006年第1期35-38,共4页 World Chinese Journal of Digestology
基金 贵州省优秀科技教育人才省长基金资助 黔科教办No.2001-06~~
关键词 丹参 重症急性胰腺炎 ET-1 大鼠 Salviae Miltiorrhizae Severe acute pancreatitis Endothelin-1 Rats
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