整合素β_1和固生蛋白Tenascin共表达与肝癌浸润转移的关系

Effect of integrin β_1 and tenascin on invasion and metastasis of hepatocellular carcinoma

目的:研究整合素β1及其配体固生蛋白Tenascin(TN)在肝细胞癌(HCC)中的表达,探讨TN和整合素β1对HCC浸润转移的影响.方法:分别采用RT-PCR和免疫组织化学法检测42例HCC、10例肝硬化和7例正常肝组织内整合素β1和TN的表达,观察其与HCC病理学特点之间的关系.结果:整合素β1在HCC中的阳性表达率显著高于非肝癌组织(c2=6.33,P<0.05);整合素β1在有包膜侵犯组、病理分级Ⅲ-Ⅳ组及有转移HCC中的表达水平明显高于无包膜侵犯组(u=3.06,P<0.01)、病理分级Ⅰ-Ⅱ级组(u=3.78,P<0.01)以及无转移组(u=3.65,P<0.01);病理分级Ⅲ-Ⅳ组、有包膜侵犯、有转移组的TN表达明显高于病理分级Ⅰ-Ⅱ级组(t=2.467,P<0.05)、无包膜侵犯(t=2.912,P<0.01)及无转移组(t=2.742,P<0.01);整合素β1阴性表达HCC中的TN表达水平明显低于整合素β1阳性和强阳性表达HCC中的TN(t=2.351,P<0.05;t=2.849,P<0.01).结论:TN和整合素β1可能协同参与了HCC的浸润和转移,检测其表达可做为判断HCC浸润转移的重要指标.

AIM： To explore the expression of Tenascin （TN） and integrinβ1 in hepatocellular carcinoma （HCC） and their effect on the invasion and metastasis of HCC. METHODS： The expression of TN and integrinβ1 were detected in 42 HCC, 10 hepatic cirrhosis, and 7 normal liver tissues by reverse transcription-polymerase chain reaction （RT-PCR） and immunohistochemical method, respectively. The relations of TN and integrinβ1 expression with the pathological features of HCC were evaluated. RESULTS： The positive expression of integrinβ1 in HCC tissues was significantly higher than that in non-HCC tissues （2 = 6.33, P 〈 0.05）. The expression of integrinβ1 in HCC with invasion of tumor envelop（u = 3.06, P 〈 0.01）, Edmondson grades Ⅲ-Ⅳ （u = 3.78, P 〈 0.01） and nonmetastasis （u = 3.65, P 〈 0.01） was significantly higher than that in HCC with no tumor envelop invasion, Edmondson grades Ⅰ -Ⅱ, and tumor metastasis. The expression of TN in HCC with Edrnondson grades Ⅲ-Ⅳ （t = 2.467, P 〈 0.05）, invasion of tumor envelop （t = 2.912, P 〈 0.01）, and metastasis （t = 2.742, P 〈 0.01） was significantly higher than that in HCC with Edrnondson grades Ⅰ- Ⅱ, no tumor envelop invasion, and non-metastasis. The expression of TN in HCC with negative expression of integrinβ1 was significantly lower than that in HCC with positive and high positive expression of integrinβ1 （t = 2.351, P 〈 0.05; t = 2.849, P 〈 0.01）. CONCLUSION： TN and integrinβ1 may play a synergic role in the invasion and metastasis of HCC, and their expression can be used as important indexes in detecting the metastasis and invasion of HCC.