抗原激活红细胞和白细胞免疫反应主干道的实验研究

Main immunological reaction route of antigen-activated red blood cells and white blood cells

目的测定抗原对红细胞和白细胞免疫反应主干道的作用。方法癌细胞(5×106/ml)和(或)卡介苗(0·1mg)或酵母菌(5×108/ml)加到全血细胞悬液0·2ml(或白细胞0·2ml)和枸橼酸抗凝的新鲜血浆0·3ml(或生理盐水0·3ml)37℃温育1h,采用免疫酶联法检测IL-8水平。分组:①抗原(癌细胞或酵母菌或卡介苗)0·2ml加入全血细胞悬液0·2ml和血浆0·3ml;②生理盐水0·2ml加入全血细胞悬液0·2ml和血浆0·3ml;③抗原0·2ml加入白细胞悬液0·2ml和血浆0·3ml;④生理盐水0·2ml加入白细胞悬液0·2ml和血浆0·3ml。结果癌细胞、卡介苗和酵母菌能激活血液免疫反应,但没有加血浆组中,这些抗原不能激活血液免疫反应,在这些抗原加入全血细胞和血浆组,IL-8激活指数(2·124±0·860)明显高于这些抗原加入白细胞和血浆组的IL-8激活指数(0·390±0·080),P<0·01。结论结果表明在血液免疫反中存在红细胞和白细胞免疫反应主干道路线图,红细胞和补体在血液免疫反应路线图中具有重要作用。

Objective To determine the effect of antigen on the main immunological reaction route of red blood cells and while blood cells. Methods Cancer cells （5 × 10^6/ml） and/or Bacillus ealmette-Guerin（BCG 0. 1mg） or yeast cells（5 × 10^8/ml） were added into 0. 2ml of whole blood cells （or 0. 2ml of white blood cells） and 0. 3ml of fresh plasma （or 0. 3ml of NS） treated by citric acid, and incubated for lh at 37℃. IL-8 level was measured by ELASA. The data could be divided into 4 groups. （1） 0. 2ml of antigen （cancer cells or yeast cells or BCG） was added to 0. 2ml of whole blood cells and 0. 3m plasma. （2）0. 2ml of NS was added to 0. 2ml of whole blood cells and 0. 3ml of plasma. （3） 0. 2ml of antigen was added to 0. 2ml of white blood cells and 0. 3ml of plasma. （4） 0. 2ml of NS was added to 0. 2ml of white blood cells and 0. 3ml of plasma Results Cancer cells, BCG and yeast cells could activate immunological reaction in blood, but could not activate immunological reaction of white blood cells in no plasma group with addition of antigen. The activation Index （2. 12±0. 860） of IL-8 in the group with addition of whole blood cells and plasma was significantly higher than that （0. 390±0. 08） in the group with addition of antigen, white blood cells and plasma （P〈0. 01）. Conclusion The results of this study indicate that there is a main immunological reaction map of red blood cells and while blood cell in blood. Red blood cell and its complement play a vital role in the regulation of the main immunological reaction route map.