脐血红细胞CD35调控白细胞CXCR4表达能力的变化

The research of change in expression of CXCR4 on leucocytes as modulated by umbilical cord erythrocyte CD35

目的探讨脐血红细胞调控白细胞CXCR4表达能力的变化。方法采用灭活癌细胞(S180,5×106/ml)0·2ml(或生理盐水)加入全血细胞(或白细胞)0·2ml和血浆0·3ml中,37℃温育1h,用流式细胞仪免疫荧光法测定红细胞CD35和白细胞(淋巴细胞、粒细胞)CXCR4分子表达量。分组:①癌细胞悬液0·2ml加全血细胞悬液0·2ml和血浆0·3ml;②生理盐水0·2ml加全血细胞悬液0·2ml和血浆0·3ml;③癌细胞悬液0·2ml加白细胞悬液0·2ml和血浆0·3ml;④生理盐水0·2ml加白细胞悬液0·2ml和血浆0·3ml。结果脐血红细胞CD35表达量在S180激活后下降不如正常人红细胞明显,而白细胞CXCR4表达量在S180激活后上升不明显;正常人全血红细胞CD35表达量在S180激活后明显下降(40·21%±11·52%vs28·65%±8·08%,P<0·01),而粒细胞CXCR4表达量明显上升(40·62%±17·89%vs64·18%±11·69%,P<0·01)。结论脐血红细胞免疫调控能力和白细胞免疫功能都明显低下。

Objective To study the changes in the expressions of CXCR4 of leucocytes as modulated by erythrocytes from cord blood. Methods 0. 2ml suspension of whole blood cells （or 0. 2ml suspension of leucocytes） was added to 0. 3ml of auto plasma, and 0. 2ml suspension of S180 （5× 10^6/ml） was added as stimuli. The suspension was incubated at 37 ℃ for 1 hour. Normal saline instead of S180 was used as control The expression changes in CD35 on erythrocytes and CXCR4 on leucocytes were determined with flow cytometry assay. The data could be grouped under four heads：（1） cancer cells 0. 2ml were added m whole blood cells 0. 2ml and plasma 0. 3ml; （2） NS 0. 2ml were added to whole blood cells 0. 2ml and plasma 0. 3ml; （3） cancer cells 0. 2ml were added to white blood cells 0. 2ml and plasma 0. 3ml; （4） NS 0. 2ml were added to white blood cells and plasma 0. 3ml. Results When activated by S180, the expression of CD35 on cord erythrocytes was decline not so low as that of adult＇ ; while the expression of CXCR4 on cord leucocytes was increased not so high as that of adults＇ When activated by S180, the expression of CD35 on adult erythrocytes was significantly decreased than that of group of not activating （40. 21±11. 52 % and 28. 65±8. 08% respectively）, while the expression of CXCR4 on adult erythrocytes was significantly increased （40. 62±17. 89% and 64. 18±11.69% respectively, P〈0. 01）. Conclusions The immune regulating ability of cord erythrocytes is lower and the immunological activity of cord leucocytes is decreased too, which provide a novel strategy for using cord blood cells as s source of stem cells in clinics.