肿瘤患者红细胞调控球蛋白变化新的自然实验体系研究

A novel experimental system for red blood cell-modulating globulin in patients with cancer

目的采用血液免疫反应路线图实验体系探讨肿瘤患者红细胞对白细胞分泌球蛋白能力的调控作用。方法将癌细胞悬液(5×106/ml)0·2ml或生理盐水0·2ml加至枸橼酸抗凝全血细胞悬液(包括红细胞和所有白细胞)0·2ml或白细胞悬液0·2ml和血浆0·3ml中,37℃水浴1h,用OlympusAU100生化测定仪检测球蛋白含量。分组:①全血细胞实验组:癌细胞0·2ml加入全血细胞悬液0·2ml和血浆0·3ml;②全血细胞对照组:生理盐水0·2ml加入全血细胞悬液0·2ml和血浆0·3ml;③白细胞实验组:癌细胞悬液0·2ml加入白细胞悬液0·2ml和血浆0·3ml;④白细胞对照组:生理盐水0·2ml加入白细胞悬液0·2ml和血浆0·3ml。结果肿瘤患者全血细胞自然实验组球蛋白激活率(0·31±0·09)明显低于正常人全血细胞自然实验组球蛋白激活率(0·39±0·14)。肿瘤患者全血细胞自然对照组红细胞对球蛋白吸附率(0·09±0·08)也明显低于正常人全血细胞自然对照组红细胞对球蛋白的吸附率(0·29±0·14)。结论血液免疫反应路线图自然实验体系用于测定红细胞对球蛋白的调控作用,方法简便,实用,肿瘤患者红细胞对球蛋白含量的调控能力明显下降。

Objective To study the effect of red blood ceils on the secretion of white blood ceils modulating globulin in patients with cancer with a new immunological reaction experimental system. Methods Cancer cetts （S180, 5×10^6/ml） or 0.2ml of NS or 0.2ml of white blood cells and 0. 3ml of plasma were added to anticoagulant suspension of white blood cells treated by citric acid and incubated for lh at 37℃. The content of globulin （g/L） was determined by Olympus AU100 detector method. 0. 2ml of cancer cells was added to 0. 2ml of whole blood cells and 0.3ml of plasma in the whole blood experimental group. 0.2ml of NS was added to 0.2ml of whole blood cells and 0. 3ml of plasma in the control group. 0. 2ml of cancer cells was added to 0. 2ml of white blood cells and 0. 3ml of plasma in the white blood cell isolation experimental group. 0. 2ml of NS was added to 0. 2ml of white blood cells and 0. 3ml of plasma in the control group. Retails Activation rate （0.31±0. 09） of globulin in whole blood cell experimental group of patients with cancer was significantly decreased compared to that （0. 39±0. 14） in normal people. Red cell adhesion rate （0. 09±0. 08） of globulin in control group was also significantly decreased compared to that （0. 29±0. 14） in normal people. Conclusion The results of our study indicate that the new experimental system can he used for detection of red blood cell-modulating globulin. The function of red blood cells in modulating globulin of patients with cancer is decreased.