HLA-G蛋白及基因在子宫内膜异位症在位和异位内膜中的表达

Expression of HLA-G mRNA and protein in ectopic and eutopic endometrium in patients with endometriosis

目的探讨人类白细胞抗原G(HLA-G)基因及蛋白在子宫内膜异位症(EM)在位和异位内膜组织中的表达及其生物学意义。方法应用免疫组化法和原位杂交法检测38例子宫腺肌症、30例卵巢子宫内膜异位症异位内膜及在位内膜、20例子宫肌瘤患者(对照组)在位子宫内膜中HLA-G蛋白和基因的表达。结果HLA-G蛋白和基因在子宫内膜异位症患者异位和在位内膜组织中的表达均明显高于对照组(P<0·01),但在异位内膜和在位内膜的表达无明显差异(P>0·05)。HLA-G蛋白在EM不同临床分期的表达无明显差异(P>0·05),在增生期和分泌期子宫内膜的表达无差异(P>0·05)。结论HLA-G的表达可能与子宫内膜异位症的发病有关。

Objective To investigate the expression of human leucocyte antigen-G （HLA-G） in the endometrium of patients with endometriosis （EM）. Methods The expression of HLA-G protein was detected in ovarian endometriosis （OEM） and adenomyosis （AM） using immunohistoehemistry method, and the expression of HLA-G mRNA was detected using in situ hybridization technique （ISH）, to compare with that in the endometrium of hysteromyoma as the control group. Results The rate of positive HLA-G expression in EM （including AM and OEM） was significantly higher than that in control group （P〈0. 01）. There was no obvious correlation between the expression of OEM and AM, or eetopie and eutopie endometrium （P〉0. 05）. The expression of HLA-G protein showed no relation with different clinical phase or different endometrial cycle. Conclusion HLA-G is overexpressed in EM, which may play certain role in the pathogenesis of the disease.