肾脏细胞凋亡在梗阻性黄疸大鼠肾脏损害中的作用及丹参的影响

Observation on apoptosis of renal cell in the rat with obstructive jaundice and salvia miltiorrhiza's effect on apoptosis

目的观察梗阻性黄疸大鼠肾脏的组织学及超微结构改变,探讨细胞凋亡在梗阻性黄疸大鼠肾脏损害中的作用及丹参的影响。方法SD大鼠100只,随机分为对照组20只,胆总管结扎组和丹参治疗组各40只;结扎胆总管建立梗阻性黄疸模型。丹参治疗组每日每只腹腔内注射1.7 g丹参注射液,对照组、胆总管结扎组每日腹腔注射等量生理盐水。3组大鼠分别于术后第1、2、3、4周剖杀,检测血清BUN和Cr的变化,观察肾脏病理组织学及超微结构改变,TUNEL法检测肾小管凋亡细胞。结果随着胆总管结扎时间的延长,大鼠血清BUN和Cr逐渐升高,肾小管上皮细胞凋亡数增加。应用丹参治疗后,肾功能和肾脏损害程度较轻,肾小管细胞凋亡数减少。结论梗阻性黄疸可以引起大鼠肾脏损害,凋亡在梗阻性黄疸大鼠肾脏功能损害中发挥重要作用,应用丹参可降低梗阻性黄疸肾脏损害程度。

Objective To investigate the function of apoptosis in the renal injury by observing change of renal pathology and ultrastructure in the rat with obstructive jaundice（OJ） and if salvia miltiorrhiza（SM） can lighten the renal dysfunction of obstructive jaundice. Methods A total of 100 adult Sprague-Dawley rats were studied. Among them, the 80 models of obstructive jaundice were established by ligating bile duct（BDL）, then divided into two groups： the OJ rats administered daily abdomen injections of SM（1.7g per rat） after operation of bile duct ligation （the SM group, n= 40） ; the OJ rats receiving only the same normal saline （the OJ group, n=40）. The other rats with sham operation receiving only normal saline （the control group, n =20）. Three groups of rats were sacrificed in groups at postoperation 1, 2, 3 and 4 week, respectively. Then serum BUN ＆ Cr were tested and renal change of histopathology and ultrastructure were observed; Apoptosis of renal tissue were assayed by TUNEL method. Results With the time of BDL extending, the value of serum BUN ＆ Cr increased; Apoptotic cells increased in renal tissue. After treatment with SM, the injury degree of renal function and histopathologic changes decreased. Conclusion Obstructive jaundice can lead to renal injury. Apoptosis had an important effect on renal function injury in the rat with obstructive jaundice. Salvia miltiorrhiza can ease the degree of renal function injury.