镓盐对骨质疏松骨细胞超微结构的影响

Study on the influence of Gallium on osteocyte ultrastructure in osteoporosis

目的探讨镓盐对骨质疏松骨细胞超微结构的影响。方法用8月龄雌性SD大鼠造模,骨质疏松组开腹切除双侧卵巢后缝合,对照组开腹切一小块脂肪后缝合。12周后,取胫骨上段骨组织,观察模型复制情况。嗣后随机分为3组:对照组与骨质疏松组正常饲养12周;硝酸镓治疗组给予硝酸镓治疗(1 mg/kg),3次/周,腹腔注射。应用脱钙骨切片观察光镜下骨形态;制作透射电镜样本,在透射电镜下观察骨细胞形态及骨细胞内部结构情况。结果对照组及硝酸镓治疗组骨细胞结构正常,细胞核染色质分布均匀,核为卵圆形;骨质疏松组部分骨细胞核呈固缩状,形态变长。对照组成骨细胞中细胞器较少,有胶原分泌;骨质疏松组成骨细胞中有大量内质网存在,有大量胶原分泌,硝酸镓治疗组成骨细胞可见有较多胶原分泌,细胞中存在大量内质网及线粒体。结论镓盐对骨质疏松引起的骨细胞超微结构的损伤有改善作用。

Objective To investigate the influence of Gallium on osteocyte ultrastructure in osteoporosis Methods A total of 66 8-month old rats were anesthetized. Oophorectomies were taken in the osteoporosis group, and in the control group a piece of fat were taken instead. 12 weeks later, 8 rats in each group were sacrificed and the tibias upside were used to observe the model duplication. After the model duplication succeeded, the residual rats were separated into 3 groups randomly： the control group, the osteoporosis group raised normally in 12 weeks and the gallium nitrate therapy group, which were injected lmg/kg gallium nitrate in the abdomen 3 times per week. Microscope was used to observe the bone form of decalcification sections. Transmission electron microscope was used to observe osteocyte form and internal structure. Results The osteocyte structures of control group and gallium nitrate therapy group were normal. The chromatins distributed symmetrical and nucleuses were oviform. Pyknosis was found in some osteocytes in osteoporosis group. There＇s few organell in osteoblast in control group. Collagen excretion was found. A lot of endoplasmic reticulums and an abundant of collagen excretion were found in osteoblasts in osteoporosis group. A lot of endoplasmic reticulums and mitochondria were found in osteoblasts in gallium nitrate therapy group. An abundant of collagen excretion was found, but less than osteoporosis group. Conclusion Gallium can alleviate the damage of osteocyte ultrastructure elicited by osteoporosis.