更年期大鼠Th1/Th2平衡与脾细胞凋亡

The changes of Th1/Th2 balance and apoptosis in splenocyte of menopausal rats

目的了解更年期大鼠脾细胞凋亡与辅助性T细胞1(T-helper l,Th1)和辅助性T细胞2(T-helper 2,Th2)型细胞因子的增龄性改变。方法应用Annexin V-FITC/PI双染色流式细胞仪测定脾细胞凋亡率;ELISA法测血清γ-干扰素(interferon-γ,IFN-γ)、白介素-4(interleukin-4,IL-4)水平。结果更年期组大鼠脾细胞凋亡率(10.3%vs.7.7%,P<0.05)、血清IFN-γ质量浓度明显高于年轻组大鼠(23.80 ng/Lvs.10.92 ng/L,P<0.01);更年期组大鼠与年轻组相比血清IL-4质量浓度有下降趋势,但差别无显著意义(0.1296 ng/Lvs.0.3094 ng/L,P>0.05);更年期组血清IFN-γ/IL-4比值明显高于年轻组(183.64vs.35.29,P<0.01),Th1/Th2平衡朝Th1漂移。结论大鼠衰老过程中Th1/Th2平衡朝Th1漂移,脾细胞凋亡增加与IFN-γ水平增高有关。

Objective To investigate the relation between the apoptosis in splenocyte and the changes of Th1/ Th2 balance in serum of menopausal rats. Methods Twenty female SD rats were divided into two groups： menopausal group（ n= 10;15 months）, young group （n= 10; 5.5 months）. The apoptosis in splenocyte of rats was measured by Annexin V-FITC/PI flow cytometric assay. The levels of IFN- γ and IL-4 in serum were measured by ELISA. Results The percentages of apoptosis cells in splenocyteof menopausal rats were significantly higher than that of young rats. The serum IFN- γ level of menopausal rats was significantly higher than that of young rats（23.80 ng/L vs. 10.92 ng/L, P〈0.01）. The level of IL-4 was similar in menopausal and young groups. Conclusion The increasing of apoptosis in splenocyte of menopausal rats is related to the increasing of IFN- γ levels with aging.