复方胰岛素凝胶剂促进大鼠皮肤溃疡愈合的初步研究

Initial experimental study of compound-insulin gel enhancing healing of skin ulcers in rats

目的:研究复方胰岛素凝胶剂对大鼠皮肤溃疡的促愈合作用,并探讨其可能的作用机制。方法:18只Wistar大鼠背部各制备4个皮肤溃疡模型,共复制溃疡72个,随机将溃疡分为复方胰岛素凝胶治疗组(A组)、凝胶基质对照组(B组)、空白对照组(C组)。以治疗后不同时间点各组溃疡面积、溃疡愈合速度及组织病理学检查评价修复效果。结果:在伤后头10天内,A组溃疡缩小最明显,愈合速度明显快于B组、C组。伤后14天,A组溃疡基本愈合,皮肤结构完整、上皮化较厚;而B、C组溃疡仍未完全愈合,皮肤结构不完整、上皮化较薄。结论:局部应用复方胰岛素凝胶剂对大鼠皮肤溃疡有促愈合作用。

Objective To investigate the effect of Compound-insulin gel on the healing of skin ulcers in rats. Methods 18 Wistar rats were used in this study.Four skin ulcers with the same depth into hypoderma were incised on the back of each rat.72 ulcers were divided into three groups：the first （group A）and the second（group B）group were treated respectively with Compound-insulin gel and Gel- Martrix.The third one（group C）were treated with nothing.The effect on ulcer healing was assessed by measurements of ulcer area,the rate of ulcer healing and histopathological changes of each group. Results In the first 10 days,Ulcer area of group A decreased most obviously in three groups,the rate of ulcer healing largest.On the 14th day, our results showed the structure of skin on healed ulcer in group A was complete;while group B and C showed non-complete-healing,for which structure of skin still wasn＇ t completely epithelized. Conclusion It was suggested that local application of Compound-insulin gel might improve healing of skin ulcers in rats.