血小板源生长因子受体在瘢痕疙瘩形成中的作用

Role of platelet-derived growth factor receptor in keloid formation

目的:观察血小板源生长因子受体α和β在瘢痕疙瘩中的表达,并与正常皮肤比较。方法:实验于2005-04/10在解放军第二军医大学长海医院中心实验室进行。切取12例瘢痕疙瘩和6例正常皮肤标本,先经原代培养为成纤维细胞,取3～6代细胞分别应用免疫细胞化学和实时定量聚合酶链反应技术,检测瘢痕疙瘩成纤维细胞和正常皮肤成纤维细胞中血小板源生长因子受体α和血小板源生长因子受体β的蛋白和mRNA表达。结果:①免疫细胞化学结果:与正常皮肤相比,瘢痕疙瘩中的血小板源生长因子受体α和血小板源生长因子受体β的染色都增强,但血小板源生长因子受体α的染色增强尤其明显;图像分析定量统计显示瘢痕疙瘩中血小板源生长因子受体α的染色阳性指数显著高于正常皮肤(2.76±0.52,0.74±0.17,P<0.01),而血小板源生长因子受体β的染色阳性指数与正常皮肤比较差异不显著(0.95±0.202,0.76±0.17,P=0.07)。②实时定量聚合酶链反应结果:瘢痕疙瘩成纤维细胞中血小板源生长因子受体α的每百万看家基因含量显著高于正常皮肤(21.73±6.51,14.41±3.37,P=0.02),而血小板源生长因子受体β的每百万看家基因含量与正常皮肤比较差异不显著(P=0.06)。结论:在瘢痕疙瘩形成过程中,血小板源生长因子受体α的蛋白和mRNA表达都显著升高,可能是其病因机制之一。

AIM： To investigate the expression of platelet-derived growth factor receptor （PDGFR）-α and PDGFR-β in keloid formation and compare with the normal skin. METHODS： The experiment was conducted in the central laboratory of Changhai Hospital, Second Military Medical University of Chinese PLA from April to October 2005. Twelve keloid samples and 6 normal skin samples were cultured primarily into fibroblasts, which were studied at passages 3- 6 with immunocytochemical and real time quantitive polymerase chain reaction to detect the protein and mRNA expression of PDGFR-α and PDGFR-β in these fibroblasts. RESULTS： ①Result of immunocytochemistry： The staining of PDGFR-α and PDGFR-β in keloid were both stronger than that in normal skin, whereas PDGFR-α was strongly stained moααre markedly; image analysis quantitive statistics demonstrated the positive index of staining of PDGFR-α in keloid group was significantly stronger than that in normal skin group （2.76±0.52,0.74±0.17 ,P 〈 0.01）, But the positive index of PDGFR-β had insignificant difference as compared with the normal skin （0.95 ±0.202, 0.76±0.17,P=0.07）. ②Result of real-time quantitative polymerase chain reaction： The real-time quantitative PCR results revealed the mRNA expression of PDGFR-ct of fibroblasts in keloid group increased significantly than that in normal skin group （21.73±6.51,14,41±3.37,P=0.02）, but which of PDGFR-β had insignificant difference as compared with the norreal skin （P=0.06）. CONCLUSION： The protein and mRNA expression of PDGFR-α elevates significantly in keloid formation, which may play a role in keloid pathogenesis.