马拉色菌与人角质形成细胞株共同培养液对培养人黑素细胞生长及酪氨酸酶mRNA表达的影响

The effects of Malassezia-keratinocytes co-culture on human melanocyte growth and tyrosinase mRNA expression

目的:观察不同马拉色菌与人角质形成细胞株共同培养液对培养人黑素细胞生长及酪氨酸酶mRNA表达的影响,探讨花斑癣的色素改变是否与马拉色菌的不同菌种有关。方法:将球形、钝形、糠秕、合轴马拉色菌分别与人角质形成细胞株共同培养,24 h后收集上清液,按一定比例加入黑素细胞培养体系中继续培养24 h:①用四甲基偶氮唑兰比色法观察对黑素细胞生长的影响;②用实时荧光定量PCR法测定黑素细胞酪氨酸酶mRNA的表达。结果:各共同培养液对黑素细胞生长无明显影响,但除球形马拉色菌与人角质形成细胞共同培养液可上调黑素细胞酪氨酸酶mRNA的表达(P<0.05)外,未见其它种马拉色菌与人角质形成细胞株共同培养液对黑素细胞酪氨酸酶mRNA的表达有影响。结论:球形马拉色菌与人角质形成细胞株共同培养液刺激黑素细胞后,能提高酪氨酸酶mR-NA的表达,该菌可能与花斑癣的色素沉着有关。

Objective： To observe the effects of the co - culture of kerationcytes with different species of Malassezia on human melanocytes growth and tyrosinase mRNA expression and to determine the correlation of pigmentary changes of pityriasis versicolor with different species of Malassezia. Methods： Malassezia globosa, M. obtusa, M. furfur or M. sympodiasis were co - cultured with keratinocytes respectively for 24 hours. The supernatants were then added to the human melanocytes culture system with different proportion for another 24 hours. The effect on melanocytes growth was measured by MTT assay, and the effect on melanocytes tyrosinase mRNA expression was determined by Real - Time PCR assay. Results： No effect on metanocytes growth was noticed in the different media. The expression of tyrosinase mRNA was increased only in the co - culture of M. globosa with keratinocytes （ P 〈 0.05 ）. Conclusion： M. globosa may be associated with hyperpigmentation of pityriasis versicolor.