Pin1在宫颈癌中过表达及其与Ki67关系的研究

The Over-expression of Pin1 and Its Association with Ki67 in Cervical Cancer

目的:探讨宫颈癌中肽基脯氨酰顺反异构酶Pin1表达、与Ki67的相关性及其临床意义。方法:应用RT-PCR和免疫组化技术检测宫颈癌组织中Pin1mRNA和蛋白表达水平及其与Ki67蛋白表达的相关性。结果:1)宫颈癌组织中Pin1mRNA表达明显高于正常宫颈组织(P<0.05);2)从正常宫颈到CIN再到浸润癌Pin1蛋白表达逐渐增强(P<0.05);3)Pin1蛋白过表达与临床分期、病理分级、淋巴结转移无明显相关(P>0.05);但宫颈腺癌Pin1表达明显高于宫颈鳞癌(P<0.05);4)Pin1过表达与Ki67表达呈显著正相关(P<0.05)。结论:1)Pin1过表达与宫颈癌细胞增殖有关,参与宫颈癌发生发展;2)Pin1过表达可作为宫颈癌诊断的辅助指标。

Objective： To study the expression status of Pin1, its association with Ki67 and their clinical importance in cervical cancer. Methods： Amplification of Pinl gene was examined by RT- PCR, and the expression of both Pinl and Ki67 protein were examined by immunohistochemistry in cervical cancer tissues. Results： 1. A significantly higher expression of Pinl mRNA was detected in cervical cancer than those in normal cervical tissues （P〈0.05）. 2. The expression of Pin1 increased progressively along with the disease process from normal cervix to CIN and to cervical cancer （P〈0.05）. 3. No significant difference of Pinl expression was found between disease stage （FIGO）, pathological grade or pelvic lymph node metastasis status （P〉0.05）. But the expression of Pin1 was significantly higher in adenocarcinoma than those in squamous carcinoma of the uterine cervix （P〈0.05）. 4. In cervical cancer, the overexpression of Pinl was positively correlated with that of Ki67 （P〈0.05）. Conclusions： The overexpression of Pinl is closely related to cancer cell proliferation or progression of cervical cancer and contributed to oncogenesis. Pinl may serve as a Potential marker for cervical cancer diagnosis.