肝细胞生长因子在正常牙髓组织中的表达

The expression of hepatocyte growth factor in human dental pulp and dental pulp cells

目的:检测肝细胞生长因子(hepatocytegrowthfactor,HGF)在体外培养的牙髓细胞及正常牙髓组织中的表达。方法:以体外培养的第4代牙髓细胞及因正畸拔出的离体牙为实验对象,应用逆转录-聚合酶联反应(real-timereservetranscriptase-polymerasechainreaction,RT-PCR)半定量技术,从基因转录水平检测体外培养的牙髓细胞中HGF及其受体C-MET基因的表达,以及外源性HGF作用下C-MET基因表达的变化;应用免疫组化法,检测体外培养的牙髓细胞及健康离体牙的牙髓组织中HGF的表达,并对其进行定位。采用SPSS10.0统计软件包对数据进行SNK-q法检验。结果:体外培养的牙髓细胞中可检测出HGFmRNA及C-METmRNA的表达,C-METmRNA的表达可被外源性HGF上调。HGF在体外培养的牙髓细胞胞质中呈阳性表达,胞核阴性表达;在健康离体牙的牙髓组织成牙本质细胞层和血管内皮细胞胞质中均呈弱阳性表达,胞核阴性表达。结论:HGF在体外培养的牙髓细胞及健康离体牙的牙髓组织中均有表达。

PURPOSE： To study the expression of hepatocyte growth factor in the dental pulp cell cultured in vitro and the human＇s healthy dental pulp. METHODS： The 4^th generation dental pulp cell cultured in vitro and the extracted tooth for orthodontic therapy were used as study targets. The gene expressions of HGF and C-MET and the expression of C-METmRNA induced by HGF were detected by RT-PCR technique ：in dental pulp cell cultured in vitro. The expression of HGF was checked by immunohistochemical method in both dental pulp tissue and dental pulp cell cultured in vitro. SPSS10.0 software package was used for SNK-q test. RESULTS： HGFmRNA and C-METmRNA could be detected in dental pulp cell cultured in vitro by real-time reserve transcfiptase-poIymerase chain reaction. HGF up regulated the expression of C-METmRNA. HGF was positively expressed in the cytoplasms of the dental pulp cell cultured in vitro whereas weak expression was seen in the odontoblast layer and vascular endothelium of the dental pulp tissue. CONCLUSION： HGF expresses by dental pulp cell cultured in vitro and dental pulp tissue of ex vivo healthy teeth.