再生障碍性贫血骨髓血管生成的研究

Bone Marrow Angiogenesis in Aplastic Anemia

本研究目的为探讨再生障碍性贫血骨髓血管生成的状况。采用免疫组织化学SP法检测32例再生障碍性贫血患者和16例正常人骨髓活检组织中血管内皮细胞标记CD34的表达,用盲法计数骨髓微血管密度(MVD)并对骨髓血管生成进行简单分级。结果表明,再生障碍性贫血患者骨髓MVD显著低于正常人(P<0.01),其中重型再生障碍性贫血和非重型再生障碍性贫血的MVD都显著低于正常人(P<0.01),而重型再生障碍性贫血MVD值低于非重型再生障碍性贫血(P<0.05)。再生障碍性贫血患者MVD与血管简单分级之间存在正相关性(r=0.64,P<0.01)。结论:再生障碍性贫血患者骨髓血管生成缺陷可能导致或加重骨髓造血障碍。

The objective of this study was to investigate the status of bone marrow angiogenesis in aplastic anemia （AA）. Bone marrow specimens from 32 patients with AA and 16 normal controls were studied. The number of bone marrow microvessels was examined by means of immunohistochemical staining for CD34. Determination of microvessel density （MVD） and angiogenesis grading were done in a blinded manner. The results showed that the bone marrow MVD in patients with AA was significantly lower than that in healthy subjects （ P 〈0.01 ）. MVD in patients with severe and moderate AA was lower than that in control group, respectively （P 〈0. 01 ）. There is significant MVD difference between severe AA and moderate AA （ P 〈 0.05 ）. Angiogenesis grade and MVD in AA were positively correlated（ r = 0.64, P 〈 0.01 ）. It is concluded that bone marrow angiogenesis in AA patients is lower than that in normal controls. Defect of angiogenesis in bone marrow may play a role resulting in or aggravating hematopoietic aplasia in patients with AA.