非霍奇金淋巴瘤患者外周血CD4^+CD25^(high)调节性T细胞研究

CD4^+ CD25^(high) Regulatory T Cells in Peripheral Blood of Patients with B Cell Non-Hodgkin's Lymphoma

本研究分析B细胞非霍奇金淋巴瘤(B-NHL)患者外周血T细胞亚群及CD4+CD25high调节T(Tr)细胞比例及其变化规律,初步探讨B-NHL免疫抑制机制,以及化疗对此免疫抑制功能的影响。采用流式细胞术检测42例初治B-NHL患者、36例化疗后达CR/PR患者及15例正常健康者(对照组)外周血CD3+、CD4+、CD8+和CD4+CD25highT细胞的比例。结果显示:B-NHL患者CD3+及CD4+T细胞比例、CD4/CD8比值均低于正常对照组,它们依次为(68.33±15.27)%,对照(72.06±9.26)%;(34.47±12.84)%,对照(42.45±9.2)%;1.36±0.26,对照1.92±0.20,但CD4+CD25highTr细胞比例明显高于正常对照组,为(4.10±1.21)%,对照(2.04±1.03)%,(P<0.001)。化疗后组CD4/CD8比值低于化疗前组(P<0.05);而外周血CD4+CD25highTr细胞比例,显著高于化疗前组和对照组(P<0.01)。结论:B-NHL初治化疗前及化疗后患者外周血CD4+CD25highTr细胞比例都显著升高,提示CD4+CD25highTr细胞可能是B-NHL患者免疫抑制的重要原因之一。

This study was aimed to analyze the proportion ot T cell subsets, CD4^＋CD25^high regulatong T Cells （Tr） in peripheral blood of B-NHL patients and thier change regularity, and to investigate the immunosuppression mechanism and influence of chemotherapy on immunosuppression function of B-NHL patients. The peripheral blood was collected from 42 patients with B-NHL , 36 patients with B-NHL who achieved partial remission （PR）or complete remission （CR） after 4-6 cycles of chemotherapy and 15 healthy controls. By using monoclonal antibodies, the blood samples were evaluated with the flow cytometry for lymphocyte subsets and Tr cells. The results showed that the proportion of CD3^＋ and CD4^＋ T cells, and the ratio of CD4/CD8 in patients with B-NHL group was significantly less than those in the healthy controls, i.e. （68.33 ± 15.27） % versus （72.06±9.26） % ; （34.47 ±12.84） % versus （42.45 ±9.2） % ; 1.36±0.26 versus 1.92 ±0.20, but the prevalence of the CD4^＋CD25^high Tr ceils was significantly higher than those in the healthy group[（4.10 ± 1.21 ） % versus （2.04± 1.03） %, P 〈 0. 001 ]. The ratio of CD4/ CD8 in chemotherapy group was lower than that in control, but the proportion of CD4^＋CD25^high Treg cells in chemotherapy group was higher than those before chemo-/radio-therapy and the control. It is concluded that the relative increase of CD4^＋CD25^high Tr cells in peripheral blood of B-NHL parients may be related to immunosuppression and tumor progression.