新疆地区维吾尔族和汉族血液淋巴细胞免疫表型参考值范围的调查

Reference Values of Blood Lymphocyte Immunophenotype in the Normal Healthy Adults of Ugyur and Han Nationalities in Xinjiang

为了建立新疆维吾尔族和汉族健康成人血液淋巴细胞免疫表型分析参考值,并比较两民族在族别,性别等方面上是否存在差异,用单克隆抗体对75例维吾尔族和104例汉族健康成人外周血进行标记,用流式细胞术进行采集分析,读取数据并对比结果,应用SPSS11.0软件进行统计学分析。结果表明:新疆地区20-50岁的维吾尔族和汉族健康成人外周血淋巴细胞亚群参考值范围:总T细胞分别为(67.85±8.97)%和(69.98±10.14)%;辅助性T细胞分别为(36.86±5.74)%和(40.07±6.10)%;抑制性T细胞分别为(26.67±6.15)%和(27.16±6.29)%;CD4/CD8比值分别为(1.46±0.47)和(1.56±0.47);NK细胞分别为(16.91±9.89)%和(12.81±7.34)%;B细胞分别为(10.09±3.33)%和(11.78±3.81)%;CD3+/HLA-DR+分别为(10.05±2.95)%和(11.27±4.98)%;CD25+细胞分别为(1.76±5.26)%和(4·10±4.30)%。两民族在辅助性T细胞水平、NK细胞、B细胞及CD25+细胞上的差异有统计学意义;维吾尔族健康人群的男性与女性在辅助性T细胞及CD4/CD8比值水平上的差异有统计学意义,而汉族男女性T细胞亚群的差别则见于抑制性T细胞和NK细胞水平;维吾尔族与汉族男性在辅助性T细胞及CD4/CD8比值上的差异有统计学意义,前者略低于后者;维吾尔族女性NK细胞显著高于汉族(P<0.01),而CD25+细胞水平较低(P<0.01)。结论:族别和性别因素可影响健康人淋巴细胞表型分布,本研究建立了新疆维吾尔族和汉族健康成人血液淋巴细胞免疫表型分布参考值,并查明两民族在族别、性别方面的差异。

This study was aimed to establish the reference values of blood lymphocyte immunophenotype in healthy adult between Ugyur and Han nationalities in Xinjiang and to compare the difference between these two nationalities in respect to nationality and gender, anticoagulated peripheral blood samples of 75 Ugyur people and 104 Han people were stained with monoclonal antibodies; the lymphocytes were analyzed by flow cytometery for the expression of lymphocyte-population beating surface markers, the data were analyzed by SPSS 11.0. The results showed that the reference ranges of blood lymphocyte subsets in Uygur and Hart adults were as follows： total T cells amounted to 67.85 ±8.97 % and 69.98 ± 10.14% respectively; helper T cell to 36.86 ± 5.74 % and 40.07 ± 6.10 % respectively; inhibitor Tcellto 26. 67±6. 15% and 27. 16 ±6.29% respectively; CD4/CD8 ratio to 1.46 ±0. 47 and 1.56 ＋0.47 respectively; NK cell to 16.91 ± 9.89% and 12.81 ± 7.34% respectively; B cell to 10.09 ± 3.33% and 11.78 ± 3. 81% respectively; CD3^＋/HLA-DR^＋ to 10.05± 2.95% and 11.27± 4.98% respectively; CD25^＋ cell to 1.76 ＋ 5.26% and 4.10 ＋4.30% respectively. The differences of those two nationalities were mainly in total T cells, NK cell, B cell and CD25 ^＋cell. Furthermore there were also some differences between male and female. There might exist differences in helper T cells, CD4/CD8 ratio between Ugyur male and female, while this difference in Han lied in inhibitor cell and NK cell. Compared to those of two nationalities, the helper T cell percentage and CD4/CD8 ratio of Uygur male were lower than those in Han male. And in female, Uygur people had higher percent of NK cell （P 〈 0.01 ）, but lower CD25 ^＋cell than those in Han＇s （ P 〈 0.01 ）. In conclusion, the nationalities and gender could influence the reference value of lymphocyte immunophenotype, the reference values of blood lymphocyte immunophenotype in the normal healthy adults of Ugyur and Han nationalities in Xinjiang were defined, and the differences between these two nationlities in respect to nationality and gender were elucidated.