机采血小板悬液在保存过程中凝血因子Ⅷ、Ⅸ生物活性的变化

Biological Activities of the Coagulation Factor Ⅷ、Ⅸ in Platelet Concentrates Collected by Platelet Apheresis During Preservation

为了研究机采血小板悬液22℃振荡保存不同时间的凝血因子Ⅷ、Ⅸ生物活性的变化,采用SYSMEXCA-1500型全自动血凝仪对用CS-3000plus血细胞分离机采集的18份血小板悬液于22℃振荡保存条件下,测定0、12、24、48、72、96、120小时7个时间段的FⅧ∶C和FⅨ∶C的活性。结果表明:机采血小板FⅧ∶C在0时活性为(100.51±44.02)%,保存12-120小时,其活性衰减了10%-40%;FⅨ∶C在0时活性为(120.93±20.50)%,保存24-120小时,其活性衰减了10%-35%。结论:机采血小板悬液于22℃振荡保存时凝血因子Ⅷ、Ⅸ仍保持有较高的生物学活性。

The study was to explore the change of coagulation factor Ⅷ and Ⅸ activities in the platelet suspension collected by platelet apheresis during storage at 22℃. 18 samples of platelet concentrates were collected by the cs-3000 plus and stored at 22℃ and then FⅧ： C, FⅨ： C activities were detected at 0, 12, 24, 48, 72, 96, 120 hours respectively by SYSMEX CA-1500. The results showed that FⅧ ： C activity was （ 100.51 ＋ 44.02 ） % at 0 hour, and then decreased dramatically to 10% - 40% of primary level from 12 to 120 hours, while FIX ： C activity was （ 120.93 ＋ 20.50） % at 0 hour and decreased to 10% -35% of primary level from 24 to 120 hours. In conclusion, FⅧ and FⅨ in the platelet concentrates stored at 22℃ could keep their biological activities at physilogically high levels.