玉米和类玉米种间杂交后代细胞学鉴定

Cytological Identification on the Interspecific Hybrid of Zea mays and Zea diploperennis

利用组成玉米异染色质钮的180-bp重复序列和TR-1元件以及45S rDNA对玉米自交系F107、GB57、二倍体多年生类玉米及其远缘杂交后代的染色体进行荧光原位杂交,确定了3种重复序列在亲本染色体上的分布;同时对远缘杂交后代进行了细胞学鉴定,通过荧光信号在染色体上的位置,证实远缘杂交后代中异源种质的染色体来源;讨论了异染色质钮重复序列对玉米和其野生种杂交后代外源染色体整合和染色体行为等方面研究的应用。

Fluorescence in situ hybridization （FISH） was carried out on mitotic chromosomes of Zea diploperennis （DP）, maize inbred lines F107, GB57 and their hybrids using knob-associated tandem repeats, 180-bp repeats and TR-1 elements, together with 45S rDNA as probes. The distribution patterns of the three repeated sequences were analyzed on parental chromosomes. The hybrids of maize and DP were identified by FISH. According to the hybridization signals on chromosome positions, the origin of foreign idioplasm was confirmed. The application using knob-associated tandem repeats as excellent chromosomal markers on detecting the integration of foreign chromosome and the chromosomal activity at meiosis was also discussed.