软骨细胞在聚乳酸支架中的体外生长行为

In vitro growth behavior of chondrocytes in poly(L-lactide) scaffolds

采用明胶和氯化钠颗粒作为致孔剂,使用溶剂浇铸/颗粒沥滤法制备了高孔隙率、孔间连通和高机械性能的聚乳酸支架, 采用软骨细胞体外培养研究了这两种多孔支架对细胞生长性能的影响．结果表明,软骨细胞在以明胶颗粒为致孔剂制备的多孔支架中的相对数量和GAG的分泌量更多,细胞的活性更高．

Gelatin or NaCI particles were employed as porogen to fabricate biodegradable poly（L- lactide） （PLLA） scaffolds by a porogenleaching technique. The scaffold has a highly porous and interconnected microstructure and good mechanical performance. The biological performance of the porous scaffolds was assessed by in vitro chondrocyte culture. A larger number of cells in the scaffolds fabricated with porogen of gelatin particles were observed after the viable cells were stained with fluorescein diacetate. Consistent higher values of MTT absorbance and GAG secretion were also determined in the scaffolds fabricated with porogen of gelatin particles than that of sodium chloride particles. The remained gelatin plays an important role for the better biological performance of the scaffold.