CK20mRNA、CD44v6和PCNA对大肠癌早期肝转移预测的研究

Study on the prediction of early liver metastasis of colorectal tumor by detection of CK20mRNA、CD44v6 and PCNA

目的:研究CK20mRNA、CD44v6和PCNA与大肠癌肝转移的关系,探讨临床预测大肠癌早期肝转移的客观指标。方法:应用荧光定量RT-PCR法检测50例大肠癌患者回流门静脉血中 CK20mRNA,同时应用免疫组织化学方法测定大肠癌癌组织中CD44v6、PCNA的表达,并与对照组比较。结果:大肠癌患者门静脉血CK20mRNA、癌组织中CD44v6、PCNA表达阳性率明显高于良性病变对照组(P<0．01)和正常对照组(P<0．01);大肠癌组织中CD44v6及PCNA的表达与门静脉中 CK20mRNA的表达有显著相关性(γ1=0．933,γ2=0.906,P<0．05);肝转移组CK20mRNA、CD44v6、 PCNA阳性表达率均高于非肝转移组(P<0.05)。结论:联合检测CD44v6、PCNA及CK20mRNA预测大肠癌肝转移,可显著提高预测的灵敏度及特异性,对于大肠癌早期肝转移监测具有很高的临床价值。

Objective：to obtain some effective objective markers used to predict the early liver metastasis of colorectal tumor,the relationship of liver metastasis of colorectal tumor with associate detection three markers such as CK20mRNA,CD44v6 and PCNA was studied.Methods：The expression of Ck20mRNA in portal venous blood from 30 colorectal cancer patients was detected by fluorescent quarto RT-PCR,and the results of CD44v6 and PCNA in colorectal cancer tissue were determined by means of immunohistochemistry,and then compared with control groups through statistics analysis.Results：The rate of positive expression of Ck30mRNA in colorectal cancer patients＇ portal venous blood was obviously superior to the level of benign pathological changes controls（P〈0.01）,and significantly higher than that of normal controls（P〈0.01）.The positive expression of liver metastasis was also clearly higher than that of no liver metastasis（P〈0.05）.The rate of positive expression of CK20mRNA in protal venous blood was evidently correlated to the expression of CD44v6 and PCNA in tumor tissue（γ1=0.933,γ2=0.906,P〈0.05）.The results of associate detection of CK20mRNA,CD44v6 and PCNA6 were closely related to the incidence of liver metatasis.Conclusion：If combined these markers of CK20mRNA,CD44v6 and PCNA to forecast liver metastasis of colorectal tumor,the sensitivity and speclallty of prediction will be notably improved,there were highly clinical values in predicting the early liver metastasis of colorectal tumor.