咪唑克生对实验性变态反应性脑脊髓炎干预作用的初步观察

Preliminary study on interventive effect of Idazoxan on experimental allergic encepha- lomyelitis in Lewis rats

目的:探讨不同剂量的咪唑克生(Ida)对Lewis大鼠实验性变态反应性脑脊髓炎(EAE)的影响。方法:采用豚鼠全脊髓匀浆(GPSCH)脚垫皮下注射,免疫Lewis大鼠复制EAE模型,采用量化评分法, 比较不同剂量组咪唑克生对EAE发病率、潜伏期、临床症状和组织学的影响。结果:对照组的发病率为 85．7％(6/7),潜伏期为(11．2±0．45)d。低剂量咪唑克生(2 mg/kg)可降低发病率37．5％(3/8),明显延长潜伏期[(13．05±0．58)d,P<0．05],减轻其临床症状和中枢神经系统(CNS)炎症细胞浸润(P< 0．05),而高剂量咪唑克生(16 mg/kg)对EAE的潜伏期、临床症状和CNS炎症细胞浸润均无影响。结论: 低剂量咪唑克生可减轻Lewis大鼠EAE的炎症细胞浸润、脱髓鞘改变及临床症状,对EAE的发病具有干预(保护)作用。推测这可能与CNS内咪唑啉2受体有关。

Objective：To investigate the effect of idazoxan with different doses on experimental allergic encephalomyelitis （EAE） in Lewis rats. Methods：The EAE in Lewis rats was induced by giving hypodermal injections in feet with spinal cords homogenate of guinea pigs （GPSCH）; The histological changes were observed under light microscope, then changes of incidence rate, latency, clinical symptoms and histology of EAE which were effected by idazoxan with different doses were compared by method of quantization. Results：The EAE in Lewis rats of contral group hadanincidence of 85.7%（6/7）） a latency of （11.2±0.45）d. Idazoxan with low dosage（2 mg/kg） could significantly decrease incidence rate of 37.5%（3/8）, and lengthen the latency（13.05 ± 0.58d P〈0.05）, ameliorate the clinical severity and infiltration of inflammatory cell in CNS of EAE（F〈 0.05）. However, Idazoxanwith high dosage （16 mg/kg） had no effect.Conclusion：Idazoxan with low dosage （2 mg/kg） can relieve the clinical severity and ameliorate inflammatory cell infiltration and demyelination of EAE induced by GPSCH in Leiws rats. It may have protective effect on EAE. It is presumed that the effect is,related to imidazoline 2 receptor in CNS.