成骨细胞复合去抗原异种松质骨再造骨组织的实验研究

Experimental study of the osteogenesis in the composite transplant about antigen-extracted massive bovine cancellous bone carrier Osteoblast Vector

目的:用胶原修饰去抗原牛松质骨载体表面,与成骨细胞复合进行体内异体移植,观察体内成骨效果,探讨其临床应用价值。方法:新鲜牛松质骨经脱脂、H2O2浸渍及部分脱钙处理,制成去抗原牛松质骨载体(antigen-extracted massive bovine cancellous bone carrier,MBC)。载体表面经胶原处理后,与兔骨膜成骨细胞复合后体外共同培养,4 w后将复合材料进行异体桡骨移植,于移植后2 w、 4 w、8 w和16 w取材,经HE染色观察。结果:植入2 w时,复合材料表面及孔内有成骨组织细胞黏附生长,材料周围可见大量软骨细胞分化,炎症反应不明显;4 w时有新骨成熟成岛状分布;8 w时有骨板形成;到16 w时,骨缺损就已完全修复,MBC部分降解,但在新骨中仍能见到残留体。结论:MBC载体表面经胶原处理后与成骨细胞的生物相容性更强,该材料复合成骨细胞后移植显示出了良好的骨诱导性和成骨活性及生物可降解性,有一定的临床应用价值。

Objective：To make up surface of MBC with collagen, increase MBC histocompatibility, and compound the cultured rabbit osteoblasts to MBC in vitro, transplant this trager to bone, to study the efficacy and explore and discuss its applied value in clinics. Methods： The bovine cancellus bone was degreased, macerated with H2O2 and decalcified to form MBC vector, surface of which was made up with collagen, then the cultured rabbit osteoblasts were combined to MBC in vitro. Two weeks later, transplant this trager to rabbits radius defects. The compound materials were observed with histological process after 2,4,8,16 weeks. Results： In second week, the cells grew and adhered in the surface of MBC, chondrocyte differentiated around MBC and also beholded mesenchyma aggregation, no obviously immunoreaction at the surrounding; in fourth week, new bone trabecule matured as islet. In eighth week, bone lamella was formed. In sixteen week, segmental bony defects were effectively repaired, degradation was seen in part of MBC, but residua of MBC were still found in the new bone. Conclusions： After the surface of the MBC is made up with collagen, the MBC have good histocompatibility with the osteoblasts. The materials show fine osteoconduction disposition,degradation nature, and definite value in clinical application.