棉铃虫齿唇姬蜂对植物挥发物和寄主性信息素腺体化合物的EAG反应

EAG responses of Campoletis chlorideae Uchida to plant volatiles and host pheromone gland compounds

测试了棉铃虫齿唇姬蜂CampoletischlorideaeUchida对58种植物挥发物和5种寄主性信息素腺体化合物的EAG反应。在100μg的刺激剂量下,棉铃虫齿唇姬蜂对C5～C7脂肪醇的EAG反应最强,碳链延长或缩短都将导致反应的降低;对脂肪醛、酮、酸和酯都有一定的反应。棉铃虫齿唇姬蜂对萜烯的EAG反应很低,对氧化萜的反应普遍升高。3种芳香化合物均可激起棉铃虫齿唇姬蜂较强的EAG反应。剂量反应曲线表明,具有EAG活性的化合物激起的反应强度与化合物的剂量呈正相关,而2种萜烯———α_蒎烯和反式石竹烯,在测定的剂量范围内EAG活性都很低。棉铃虫齿唇姬蜂对寄主性信息素腺体化合物也有EAG反应,用100μg和1000μg的寄主性信息素刺激,雄蜂的反应可比雌蜂的反应高出1倍。对植物挥发物在棉铃虫齿唇姬蜂寻找寄主中的作用进行了讨论。

Electroantennogram （EAG） responses of the generalist parasitoid Campoletis chlorideae Uchida to a range of plant volatiles and host pheromone gland compounds were investigated. The EAG responses were maximal for the C5 - C7 alcohols, and declined as the carbon chain-length of compounds increased or decreased. Aliphatic aldehydes, acetones, acids and esters could also trigger significant EAG responses. All monoterpene hydrocarbons elicited low EAG response, while oxygenated monoterpenes elicited relatively high responses. Three aromatic compounds elicited significant EAG responses. Compounds that showed significant EAG responses at dose of 100μg elicited dose-dependent EAG response, while a-pinene and （E）caryophyllene evoked little responses at all tested dosages. All tested host pheromone gland compounds elicited significant EAG response at the dosage of 100μg and 1 000μg. Tested with 100μg and 1 000μg of host pheromones, the male parasitoids showed responses two times as high as that of the female. The role of plant volatiles in host location behavior of the parasitoid was discussed.