大鼠精索静脉曲张模型附睾中丙二醛、总抗氧化物及唾液酸含量的改变及意义

Effects of Experimental Varicocele on Methylenedioxyamphetamine,Total Antioxidants Content and Sialic Acid of the Epididymis in Adolescent Rats

目的:探讨大鼠左侧精索静脉曲张后同侧附睾组织中丙二醛(MDA)、总抗氧化物(TAC)和附睾管腔中唾液酸(SA)含量的改变及意义。方法:用8只雄性SD大鼠建立左侧精索静脉曲张模型,另取8只SD大鼠作为对照组。硫代巴比妥酸法和铁离子还原法分别检测附睾组织中MDA和TAC含量;5甲-基苯二酚法检测附睾腔液中SA含量。结果:大鼠左侧精索静脉曲张模型建立后的第7 d,同侧附睾组织中MDA和TAC含量,模型组与对照组相比,差异有显著性(P<0.005);附睾管腔液中SA含量,模型组明显低于对照组,差异有显著性(P<0.005)。结论:大鼠左侧精索静脉曲张会导致同侧附睾组织中活性氧增加,TAC含量下降,进而影响到附睾上皮细胞合成和分泌SA的功能。

Objective： To explore the changes of methylenedioxyamphetamine （MDA）, total antioxidants content （TAC） and sialic acid （SA） from the unilateral epididymis of experimental varicocele in adolescent rats, and to illuminate the effects of varicocele on unilateral epididymis epithelium. Methods： Experimental left varicocele model of 16 adult male Sprague-Dawley rats were established by partial ligation of left renal vein. The epididymis were collected for detecting the content of MDA, TAC and SA by using spectrophotometry. Results： There was statistically significant differences in the contents of three substances between experimental varicocele and sham-operated groups. Conclusion ： The content of MDA, TAC and SA will change and the sialic acid-secreting-function of unilateral epididymis will be injured because of varicocele.