神经干细胞移植对AD鼠基底前脑NOS阳性神经元的影响

Effects of NOS-positive Neurons of Basal Forebrain of AD Rat after Neural Stem Cells Transplantation

目的探讨神经干细胞(neural stem cells,NSCs)移植对老年性痴呆鼠基底前脑一氧化氮合酶(NOS)阳性神经元的影响。方法切断成年SD大鼠左侧穹窿海马伞(fimbria-fornix,FF),于基底前脑行神经干细胞移植,4周后行组织化学染色结合图像分析技术观察各组大鼠基底前脑NOS阳性神经元数量和形态学参数的变化。结果损伤后大约1个月,损伤侧基底前脑内侧隔核(MS)和斜角带垂直支(VDB)内可观察到NOS阳性神经元明显减少,分别为正常组的35.5%和55.8%,(与正常组相比P<0.01);移植组NOS阳性神经元数恢复到正常组的74.7%和95.7%,(与损伤组相比P<0.01)。细胞形态学参数提示移植组NOS阳性神经元中含部分中等大小的未成熟细胞。结论神经干细胞移植治疗,对AD模型鼠基底前脑MS、VDB的NOS阳性神经元有明显的补充和保护作用。

Objective To investigate the effects of NOS-positive neurons of basal forebrain of rats with Alzheimer＇s disease （AD） after neural stem cells （NSCs） transplantation. Methods Adult SD rats with left fimbria-fomix （FF） transection received neural stem cells transplanted in the basal fore brain. After 4 weeks, morphologic data and percentages of NOS-positive neurons were measured in basal forebrains by using histochemical method combined with technique of micromeasure and image analysis. Results About 1 month after FF lesion, 35.5% and 55.8% NOS-positive neurons were survived in medial septum （MS） and vertical diagonal band （VDB） of the lesion side of basal forebrain, （P〈0. 01, compared with the normal control group）. After the transplantation of NSCs, 74. 7%/95. 7% NOS-positive neurons were survived in MS/VDB, （P 〈 0.01 compared with the lesion group）. The morphologic data of NOS-positive neurons indicated that there were some premature cells among them in grafting group. Conclusions The results suggest that the NOS-positive neurons in MS and VDB of basal forebrain of AD rats could be improved and protected after treatment with neural stem cells grafting.