白芍总苷对胶原性关节炎大鼠滑膜细胞的作用及机制

Effect and mechanism of action of total glucosides of paeony on synoviocytes from rats with collagen-induced arthritis

目的研究白芍总苷(TGP)对胶原性关节炎(C IA)大鼠滑膜细胞的作用及机制。方法采用鸡II型胶原诱导大鼠C IA模型,胶原酶和胰蛋白酶消化法分离培养大鼠滑膜细胞,透射电镜观察滑膜细胞超微结构的变化,MTT法检测滑膜细胞的增殖能力,滑膜细胞培养上清液中IL-1活性的测定采用小鼠胸腺细胞增殖法,TNFα和PGE2含量的测定采用放射免疫测定法。结果TGP能有效改善C IA大鼠滑膜细胞超微结构的变化,抑制其过度的增殖反应和产生IL-1,TNFα和PGE2的水平。结论TGP对C IA大鼠功能亢进的滑膜细胞具有明显的抑制作用,其作用机制可能与其抑制滑膜细胞的过度增殖和分泌能力有关。

Aim To study the effect and mechanism of action of total glucosides of paeony （TGP） on synoviocytes from rats with collagen-induced arthritis （CIA）. Methods used to induce CIA in rats. Synoviocytes were separated by incubation with Chicken type II collagen was collagenase and trypsin, and its ultrastructural changes were observed under transmission electron microscope. Synoviocyte proliferation was determined by 3- （4,5-dimethyhhiazal-2yl） 2,5- diphenyhetrazoliumbromide （MTT） assay, and IL-1 activity in synoviocytes supernatant was measured by thymocyte proliferation assay. TNFα and PGE2 produced by synoviocytes were determined by radioimmunoassay. Results TGP was shown to protect CIA rats against the ultrastructural damages of synoviocytes. Meanwhile, TGP also suppressed the excessive synoviocyte proliferation and over-production of IL-1, TNFα and PGE2. Conclusion TGP has inhibitory effect on hyperfunctional synoviocytes of CIA rats and its mechanism of action may be related with the inhibition of abnormal proliferation and secretion of synoviocytes.