胚胎培养液和体外操作环境对IVF-ET妊娠率的影响

Influence of embryos culture environment on the pregnancy rate

目的:探讨胚胎培养液和体外操作环境对体外受精-胚胎移植(IVF-ET)妊娠率的影响,以便建立更适宜的胚胎体外培养体系。方法:对78例(79个周期)不孕症患者的临床资料进行回顾性分析。79个IVF-ET周期患者分为2组,Ⅰ组21个周期使用单纯EBSS培养液,无37℃恒温、保湿、维持pH的设施,Ⅱ组58个周期使用HTFI、VC-ONE序列培养基,且配备37℃恒温、保湿、维持pH恒定的设施,比较2组的受精率、卵裂率、优质胚胎率、着床率和临床妊娠率。结果:Ⅰ组与Ⅱ组的受精率、卵裂率、优质胚胎率分别为74.88%、76.39%,72.46%、76.01%,38.00%、41.86%,差异均无统计学意义(P>0.05),而胚胎着床率、临床妊娠率分别为2.32%、19.75%,9.52%、44.82%,Ⅱ组显著高于Ⅰ组,差异有统计学意义(P<0.01)。结论:序列培养基与37℃恒温、保湿、维持pH设施结合应用,改善了胚胎的潜在质量,显著提高了IVF的妊娠率。

Objective： To investigated the Influence in vitro embryos culture environment on the pregnancy rate so that to establish the more suitable culture system of embryos. Methods： Two culture media were used in two groups. The first was HTF and IVC ONE media culture system （maintaining 37℃, relative humidity, 5%CO2） which was used in 58 IVF-ET cycles, and the second was EBSS media system （not maintaining 37℃, relative humidity, 5%CO2） in 21 cycles. The fertilization rate, cleavage rate, good embryo rate, embryo imbed rate and pregnancy rate were compared between these two groups. Results： There were significantly higher embryo imbed rate and pregnancy rate （19.75%, 44.82% respectively） used HTF and IVC-ONE media culture system group as compared those in EBSS media system group （2.32%, 9.52% respectively）. There were no significant differences in fertilization rate, cleavage rate, good embryo rate between two groups （76.39%, 76.01%, 41.86% and 74.88%, 72.46%, 38% respectively）. Conclusion： HTF and IVC-ONE media culture system can improve the pregnancy rate significantly.