摘要
目的面向中药制药过程,提出一种用近红外(NIR)漫反射光谱快速测定复方丹参滴丸有效成分的新方法。方法采用HPLC为对照分析法测定滴丸中3种酚酸类有效成分:丹参素(danshensu,Da)、原儿茶醛(protocatechuic aldehyde,Pra)和丹酚酸B(salvianolic acid B,Sal B)含量。运用偏最小二乘(PLS)法建立NIR光谱与3成分HPLC分析值之间的多元校正模型,对未知样本进行含量预测。结果校正模型相关系数分别为0.994 2,0.996 5和0.971 4,验证集相对预测误差均方根(RSEP)分别为5.916 1%,9.291 7%和12.423 2%。结论本法操作简便、快速无损,可用于中药复方制剂有效成分快速检测。
OBJECTIVE To develop a novel method for fast determination of three active compounds in compound Danshen dropping pills (DSDP) with near infrared (NIR) spectroscopy. METHODS HPLC was used as the reference method to determine the contents of Danshensu (Da), protocatechuic aldehyde (Pra) and salvianolic acid B (Sal B) in DSDP. NIR diffuse reflectance spectra of the samples were, collected in range of 4 000 - 10 000 cm^- 1. Multivariate calibration models based on Pl S algorithm were developed to correlate the spectra and the corresponding values determined by the reference methods. RESULTS The corelation cnefficients of the calibration models were 0.994 2,0.996 5 and 0.971 4,and the RSEP for Da,Pra and Sal B were 5.916 1% ,9.291 7% and 12.423 2% ,respectively. CONCLUSION The proposed method is fast and nondestructive, and it provides a novel efficient and environmental friendly approach for the fast determination of the active components in Chinese medicine.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2006年第3期226-229,共4页
Chinese Pharmaceutical Journal
基金
国家自然科学基金重大研究计划重点项目(90209005)
关键词
近红外漫反射光谱
偏最小二乘法
定量分析
中药复方制剂
复方丹参滴丸
NIR diffuse reflectance spectroscopy
PLS
quantitative analysis
Chinese traditional medicine compound
compound Danshen dropping pills