基因芯片对尖锐湿疣患者HPV的检测和基因分型

Detection and Classification of the Human Papillomavirus Genotype in Condyloma Acuminata with Gene-chips

目的:探讨深圳地区尖锐湿疣(CA)患者人乳头瘤病毒(HPV)的基因型别感染情况及临床意义。方法:采用基因芯片法检测158例CA患者病损组织或脱落细胞中HPV的23种基因型的感染率。结果:158例CA患者标本中检出HPV阳性156例,总的阳性检出率为98.7%。其中单一型别阳性检出率为72.2%,包括5种低危型别HPV6、11、42、43、44总阳性检出率为58.2%和9种高危型别HPV16、18、31、33、35、45、55、58、59总阳性检出率为14.6%,单一型别感染中以HPV11(33.5%)型为主,其次是HPV6(20.3%)型;混合型感染阳性检出率为27.2%,其中以HPV6+11(11.4%)型感染为主,其次是HPV16+18(8.9%)型。结论:HPV11、6、6+11、16+18型感染为CA的主要致病型别,基因芯片是一种比较适合临床HPV分型检测的、敏感性高和特异性好的诊断方法。

Objective： To study the genotypes of human papillomavirus （ HPV ） and its clinical characteristics in condyloma acuminata（CA） in Shenzhen city. Method： Gene -chips technique was used to determine 23 types of HPV genotype in specimens of dropping cells or lesions from 158 patients with CA. Result： There were 156 positive cases in 158 patients with CA , The total positive rates was 98.7%. and the positivity rates of single types infection with HPV was 72.2% ,include the detecting total rates of 5 low risk HPV genotypes 6,11,42,43,44 were 58.2% ,while the detecting total rates of 9 high risk HPV genotypes 16,18,31,33,35,45,55,58,59 werel4.6% ,The predominant type from single infection with HPV was HPV11（33.5% ）, secondly HPV6 （20.3%）. The positivity rates of mixed infection with HPV was 27.2%, the predominant type from single infection with HPV was HPV6 ＋ 11 （ 11.4% ）, secondly HPV16 ＋ 18 （8. 9% ）. Conclusion： HPV11,6,6 ＋ 11,16 ＋ 18 are the main genotype in the pathogenesis of CA and Gene - chips method is a high sensitivities and specificities method in detection and typing of HPV.